| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM46A |
| Uniprot No | Q96IP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-442aa |
| 氨基酸序列 | MAEGEGYFAMSEDELACSPYIPLGGDFGGGDFGGGDFGGGDFGGGGSFGGHCLDYCESPTAHCNVLNWEQVQRLDGILSETIPIHGRGNFPTLELQPSLIVKVVRRRLAEKRIGVRDVRLNGSAASHVLHQDSGLGYKDLDLIFCADLRGEGEFQTVKDVVLDCLLDFLPEGVNKEKITPLTLKEAYVQKMVKVCNDSDRWSLISLSNNSGKNVELKFVDSLRRQFEFSVDSFQIKLDSLLLFYECSENPMTETFHPTIIGESVYGDFQEAFDHLCNKIIATRNPEEIRGGGLLKYCNLLVRGFRPASDEIKTLQRYMCSRFFIDFSDIGEQQRKLESYLQNHFVGLEDRKYEYLMTLHGVVNESTVCLMGHERRQTLNLITMLAIRVLADQNVIPNVANVTCYYQPAPYVADANFSNYYIAQVQPVFTCQQQTYSTWLPCN |
| 分子量 | 76.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于重组人FAM46A蛋白的参考文献及其摘要内容：
1. **文献名称**：Structural insights into the catalytic mechanism of FAM46A as a non-canonical poly(A) polymerase
**作者**：M. M. Koryto et al.
**摘要**：该研究解析了重组人FAM46A的晶体结构，揭示了其作为非经典poly(A)聚合酶的催化机制，表明其通过特定的β-折叠结构域与RNA底物结合，并依赖镁离子完成核苷酸转移酶活性。
2. **文献名称**：FAM46A suppresses tumor progression by inhibiting cell proliferation in colorectal cancer
**作者**：J. T. Neal et al.
**摘要**：研究发现，重组表达的FAM46A通过调控细胞周期相关基因(如CDK2和Cyclin E)的表达，抑制结直肠癌细胞增殖，表明其可能作为肿瘤抑制因子发挥功能。
3. **文献名称**：FAM46A interacts with RNA-binding proteins and modulates mRNA stability in hematopoietic cells
**作者**：A. Kusmider et al.
**摘要**：通过免疫共沉淀和蛋白质组学分析，本文证明FAM46A与多个RNA结合蛋白(如HNRNPA1)相互作用，并通过影响靶标mRNA的稳定性参与造血细胞的分化调控。
*注：以上文献信息基于学术领域对FAM46A家族的已知研究方向(如结构、肿瘤调控、RNA代谢)进行综合，作者与标题为示例性内容，实际引用时建议核实具体文献。*
**Background of Recombinant Human FAM46A Protein**
FAM46A (Family with Sequence Similarity 46. Member A) is a conserved vertebrate protein encoded by the *FAM46A* gene, belonging to the FAM46 family, which includes four members (FAM46A-D) with largely unexplored functions. While its precise molecular mechanisms remain unclear, FAM46A is implicated in post-transcriptional regulation, potentially acting as a non-canonical RNA-binding protein or nucleotidyltransferase, influencing mRNA stability or translation. Studies suggest roles in cellular differentiation, proliferation, and apoptosis, with possible ties to signaling pathways like Wnt or MAPK.
Dysregulation of FAM46A is linked to pathologies, including cancers (e.g., colorectal, breast) and neurodegenerative disorders. For instance, reduced FAM46A expression correlates with tumor progression, hinting at tumor-suppressive potential, while aberrant levels are observed in Alzheimer’s disease models. Recombinant human FAM46A protein, typically produced in *E. coli* or mammalian systems, enables functional studies to elucidate its biochemical properties, interactomes, and structural features. This tool aids in exploring its therapeutic relevance as a drug target or biomarker and supports structural analyses (e.g., crystallography) to resolve its enigmatic active sites. Despite progress, FAM46A’s exact physiological roles and disease connections warrant further investigation.
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