| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM46B |
| Uniprot No | Q96A09 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-424aa |
| 氨基酸序列 | MPSESGAERRDRAAAQVGTAAATAVATAAPAGGGPDPEALSAFPGRHLSGLSWPQVKRLDALLSEPIPIHGRGNFPTLSVQPRQIVQVVRSTLEEQGLHVHSVRLHGSAASHVLHPESGLGYKDLDLVFRVDLRSEASFQLTKAVVLACLLDFLPAGVSRAKITPLTLKEAYVQKLVKVCTDSDRWSLISLSNKSGKNVELKFVDSVRRQFEFSIDSFQIILDSLLLFGQCSSTPMSEAFHPTVTGESLYGDFTEALEHLRHRVIATRSPEEIRGGGLLKYCHLLVRGFRPRPSTDVRALQRYMCSRFFIDFPDLVEQRRTLERYLEAHFGGADAARRYACLVTLHRVVNESTVCLMNHERRQTLDLIAALALQALAEQGPAATAALAWRPPGTDGVVPATVNYYVTPVQPLLAHAYPTWLPCN |
| 分子量 | 73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM46B蛋白的3篇参考文献及其摘要概括(基于公开信息推测，实际文献需核实)：
1. **文献名称**：*Structural insights into the nucleotide transfer mechanism of human FAM46B*
**作者**：Smith J, et al.
**摘要**：本研究解析了重组人FAM46B蛋白的晶体结构，证实其具有保守的核苷酸转移酶活性，并通过体外实验证明其偏好poly(A) RNA底物，推测其在真核细胞mRNA代谢中起调控作用。
2. **文献名称**：*FAM46B suppresses tumor progression by inhibiting cell proliferation in colorectal cancer*
**作者**：Wang L, et al.
**摘要**：研究通过重组表达FAM46B蛋白，发现其过表达显著抑制结直肠癌细胞增殖并诱导细胞周期停滞，提示FAM46B可能作为肿瘤抑制因子通过调控关键信号通路发挥作用。
3. **文献名称**：*Recombinant FAM46B interacts with viral RNA and modulates host antiviral response*
**作者**：Chen H, et al.
**摘要**：利用大肠杆菌系统表达纯化重组FAM46B，发现其直接结合RNA病毒基因组，并通过增强宿主干扰素信号通路抑制病毒复制，表明其在天然免疫中的潜在功能。
**注意**：以上内容为示例，实际文献需通过数据库(如PubMed、Google Scholar)检索确认。FAM46B研究相对较少，建议结合其同源蛋白(如FAM46A/C)或关键词“TENT5C”(更新命名)扩展搜索。
**Background of Recombinant Human FAM46B Protein**
FAM46B (Family with sequence similarity 46. member B) is a conserved eukaryotic protein belonging to the FAM46 family, implicated in diverse cellular processes, particularly RNA metabolism. Although its exact molecular function remains under investigation, FAM46B is hypothesized to act as a non-canonical poly(A) polymerase or RNA-binding protein, potentially regulating mRNA stability, translation, or post-transcriptional modifications. Structurally, it contains a conserved nucleotidyltransferase (NTase) domain, suggesting enzymatic activity in nucleotide addition or RNA modification.
Studies link FAM46B to critical physiological and pathological roles. It is expressed in various tissues, with higher levels observed in the brain, testes, and immune cells, implying tissue-specific regulatory functions. Dysregulation of FAM46B has been associated with diseases, including cancers (e.g., hepatocellular carcinoma, gliomas) and neurodegenerative disorders, where its altered expression may influence cell proliferation, apoptosis, or stress responses.
Recombinant human FAM46B protein is generated via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its recombinant form enables exploration of biochemical properties, interactomes, and mechanisms in RNA-related pathways. Current research focuses on clarifying its role in cellular RNA biology and disease pathogenesis, with potential applications in therapeutic targeting or biomarker development. However, gaps persist in understanding its precise substrates, regulatory networks, and disease-specific contributions, necessitating further mechanistic studies.
×
