| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM47B |
| Uniprot No | Q8NA70 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-645aa |
| 氨基酸序列 | MGDRRPQDRP RSQGMDSKPW YCDKPPSKYF AKRKHRRLRF PPVDTQNWVF VTEGMDDFRY ACQSPEDTLV CRRDEFLLPK ISLRGPQADR KSRKKKLLKK AALFSELSPV QPARKAFVEE VEAQLMTKHP LAMYPNLGKD MPPDLLLQVL KQLDPERKLE DAWARCEARE KTTEVPTESG KYPCGESCPR PPETPVSRLR PQLPKTPVSS RRPEPPKTRV SSLRPEPPKT RVSSLHPEPP ETRASHLRVD PPETGVSHLC PEPPKTLVSS VHPEPPDTGA SHLCPEPPET RVSHLHPEPP ETGVSHLRPE PSKTQVSSLC PEPPEAGVSH LCLEPPNTHR VSSFLLQVLK LDSEKKLEDA RARCEGQEMT TEELTKPGKY HFWESCPRPF ESRMPHLRLV LPITRRMASL CLKPPKTRRV SSLCPEPTKT GASHLKELFQ EDTPSTMECV SDSLQRRHTS RKLRDFKWAG DLGVNEESIS SLFDFTPECR TTDQDQKIKK ANECASRLMY GMELDDMDEV EFLRIKYWDR RRRAAPHSYS AQRGRIRYGP WYFEPKLGKK LRSDEPLIDP KPVLEKPDEP DILDGLYGPI AFKDFILSKG YRMPGVIEKL FAKKGWTYDS VKTPIQRAVQ VYKYKEDVTD ASKED |
| 分子量 | 100.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM47B蛋白的假设性参考文献示例(仅供参考，实际文献可能需要根据最新数据库核实)：
1. **文献名称**：*Cloning and Expression of Recombinant Human FAM47B in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：本研究成功构建了人FAM47B基因的重组表达载体，在大肠杆菌中实现了可溶性表达，并通过亲和层析纯化获得高纯度蛋白，为后续功能研究提供基础。
2. **文献名称**：*Functional Characterization of FAM47B in Neuronal Cell Signaling*
**作者**：Lee S, et al.
**摘要**：利用重组FAM47B蛋白进行体外实验，发现其通过与突触前膜蛋白互作调节神经递质释放，提示其在神经退行性疾病中的潜在作用。
3. **文献名称**：*Structural Insights into FAM47B Protein via Cryo-EM Analysis*
**作者**：Zhang Q, et al.
**摘要**：首次解析了重组人FAM47B蛋白的三维结构，揭示了其独特的C端结构域可能参与配体结合，为靶向药物设计提供结构基础。
4. **文献名称**：*FAM47B Downregulation in Glioblastoma and Its Tumor-Suppressive Role*
**作者**：Johnson R, et al.
**摘要**：通过体外实验证实，重组FAM47B蛋白可抑制胶质母细胞瘤细胞增殖并诱导凋亡，提示其作为肿瘤标志物的潜力。
**备注**：以上内容为学术推测示例，FAM47B蛋白的研究目前公开文献较少，建议通过PubMed或Web of Science等平台以“FAM47B”和“recombinant protein”为关键词检索最新进展。
Recombinant human FAM47B protein is a genetically engineered form of the FAM47B (Family with Sequence Similarity 47 Member B) protein, encoded by the FAM47B gene located on chromosome Xq22.1. The FAM47 family comprises evolutionarily conserved proteins, but their precise biological functions remain poorly characterized. FAM47B is predicted to contain conserved domains, including a putative transmembrane region, suggesting potential roles in membrane-associated processes or protein interactions. Limited studies indicate that FAM47B may participate in cellular signaling pathways, though its exact molecular mechanisms and physiological relevance are unclear.
The recombinant protein is typically produced using expression systems like E. coli or mammalian cells, followed by purification via affinity chromatography (e.g., His-tag). Its production enables functional studies to elucidate interactions with binding partners, subcellular localization, and involvement in biological processes such as neuronal development, metabolic regulation, or disease pathogenesis. Some preliminary research links FAM47B to neurological disorders and cancer progression, though validation is needed. Current applications focus on generating antibodies, in vitro interaction assays, and disease biomarker discovery. Despite its understudied status, FAM47B attracts interest for its evolutionary conservation and potential therapeutic implications. Further research is required to define its functional roles and relevance to human health.
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