| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM47A |
| Uniprot No | Q5JRC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-791aa |
| 氨基酸序列 | MGDQRLQDWL RSPGMDSKPW YCNKRPSKCF AKCKHRRLRF PPMDTQNWVF VKEGMDDFRY GCPSPEDTLV CRRDEFLLPK ISLRGPQADP KSGQKKLLKK AALFSKLSPA QLARKAFVEQ VEAQLMAKHP LAMYPNLGED MPPDLLLQVL KHLDPERELE DAWACCETQE KTTEVPTEPG KHPCGEFCLK PPETPVSHLL PEPPETGVSH LSPEPPKTPV SSLRPEPPET GVSHLRPEPP ETGVSHIRPG PPITRRRSSL LRQLLKLDSE RKLEDARAPC EGREKTTDEP TEPGKYPCGK FCPRPFETPL SHLRQEPPKT PVSSLRPEPP ETGESHLRLE HSKTRRGSSL RSEPSETGVS RLRLAPPKTR RGSSLHAEPS KTGVSHLSPE PPKTEVSHLH PVPPKTGVCH LRLEPPDTSQ VSNLLLYILK VLDSGRTLKD VWDRCEARVK KTKEPTEPHK SPCGEPCLQP PETQVSHPHP EHPKTRRRSS LHSQPPKTRR TSSLRSEPPK TRRTSSLRSE PPKTRRTSSL GPEPPKTRRV SSLRPELPKS RRVSSLHPEP PKAPESHQFS EPPKIRASYI KELLQEDTPS TKECVSDSLQ YRYTSEKLRE FFKWAGDLGA DEESIRNLFD FTPKYRATHE DQKFKKVKEC SSELKYSMEL DEKDEDKFFS QEKYWGRKFH TPSNSYTAQR VKMKYGAWYL KPKLWKKLRS DEPLIDPKLL LKKPDEPDVL DDLYGPIAFK DFILSKGYEM PGIIQRLFAR RGWTYDSVKT PIQRAMIFYK YKEIVEASEE D |
| 分子量 | 90.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM47A蛋白的示例参考文献(注：FAM47A研究有限，以下内容为假设性示例，建议通过PubMed或Google Scholar验证真实文献)：
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1. **标题**：Recombinant Human FAM47A Expression in HEK293 Cells and Its Role in Neuronal Development
**作者**：Smith J, et al.
**摘要**：本研究成功在HEK293细胞中表达并纯化了重组人FAM47A蛋白，发现其通过与Synaptotagmin-1相互作用调控突触囊泡运输，提示其在神经元发育中的潜在功能。
2. **标题**：Structural Characterization of FAM47A Protein and Its Interactions with Ubiquitin Ligases
**作者**：Li Y, et al.
**摘要**：通过X射线晶体学解析了重组FAM47A的结构，揭示其N端结构域与E3泛素连接酶的相互作用，为研究其在蛋白质降解通路中的作用提供依据。
3. **标题**：FAM47A Knockdown Impairs Mitochondrial Function and Cell Viability in Cancer Cells
**作者**：Garcia R, et al.
**摘要**：利用重组FAM47A蛋白进行功能补偿实验，证明其在维持线粒体膜电位和抑制肿瘤细胞凋亡中的关键作用，可能与癌症进展相关。
4. **标题**：Development of a High-Yield Prokaryotic Expression System for Recombinant FAM47A
**作者**：Chen L, et al.
**摘要**：优化了FAM47A在大肠杆菌中的可溶性表达条件，并通过镍柱层析获得高纯度蛋白，为后续抗体开发及功能研究奠定基础。
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建议通过关键词 **"FAM47A recombinant protein"** 或 **"FAM47A function"** 在学术数据库中检索最新文献，部分研究可能以预印本或早期成果形式发表。
The FAM47A protein, a member of the FAM47 family in humans, is a poorly characterized protein with limited functional information available in current research. It is encoded by the FAM47A gene located on the X chromosome (Xq22.1). While its exact biological role remains unclear, FAM47A shares homology with other FAM47 family members, such as FAM47B and FAM47C, which are hypothesized to participate in cellular communication, membrane trafficking, or intracellular signaling pathways. Structural predictions suggest FAM47A may contain conserved transmembrane domains or signal peptides, implicating potential localization to cellular membranes or secretion pathways.
Recombinant human FAM47A protein is engineered through molecular cloning, typically expressed in bacterial, yeast, or mammalian systems to ensure proper post-translational modifications. Purification methods often involve affinity tags (e.g., His-tag) for isolation. This recombinant form enables functional studies, such as elucidating interaction partners via co-immunoprecipitation or yeast two-hybrid assays, and exploring its role in physiological or pathological contexts. Limited studies suggest potential associations with neurodevelopmental processes or cancer, though mechanistic insights are lacking. Current applications include antibody production, cell-based assays, and as a reference in -omics studies to map protein networks. Further research is needed to define its precise molecular functions and therapeutic relevance.
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