| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHRS13 |
| Uniprot No | Q6UX07 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MTALELARRGARVVLACRSQERGEAAAFDLRQESGNNEVIFMALDLASLASVRAFATAFLSSEPRLDILIHNAGISSCGRTREAFNLLLRVNHIGPFLLTHLLLPCLKACAPSRVVVVASAAHCRGRLDFKRLDRPVVGWRQELRAYADTKLANVLFARELANQLEATGVTCYAAHPGPVNSELFLRHVPGWLRPLLRPLAWLVLRAPRGGAQTPLYCALQEGIEPLSGRYFANCHVEEVPPAARDDRAAHRLWEASKRLAGLGPGEDAEPDEDPQSEDSEAPSSLSTPHPEEPTVSQPYPSPQSSPDLSKMTHRIQAKVEPEIQLS |
| 分子量 | 62.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DHRS13蛋白的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**：*Expression and Purification of Recombinant Human DHRS13 in Escherichia coli*
**作者**：Zhang Y, et al.
**摘要**：该研究报道了人源DHRS13蛋白在大肠杆菌系统中的重组表达与纯化方法，优化了诱导条件并证实其酶活性，为后续功能研究提供基础。
2. **文献名称**：*Crystal Structure Analysis of Human DHRS13 Reveals Substrate-Binding Mechanism*
**作者**：Li X, et al.
**摘要**：通过X射线晶体学解析了DHRS13的三维结构，明确了其催化活性位点和底物结合区域，揭示了其作为短链脱氢酶的分子机制。
3. **文献名称**：*DHRS13 Modulates Retinoid Metabolism via Catalytic Activity in Cellular Assays*
**作者**：Smith JP, et al.
**摘要**：功能研究表明，重组DHRS13通过催化类视黄醇代谢物的氧化/还原反应参与细胞分化调控，提示其在脂质代谢中的潜在作用。
4. **文献名称**：*DHRS13 Overexpression in Colorectal Cancer and Its Role in Cell Proliferation*
**作者**：Wang L, et al.
**摘要**：研究发现DHRS13在结直肠癌中异常高表达，通过重组蛋白实验证实其可通过影响视黄酸信号通路促进癌细胞增殖。
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**注意事项**：
- 以上为模拟示例，实际文献需通过**PubMed**、**Web of Science**或**Google Scholar**等平台检索(关键词：recombinant DHRS13. human DHRS13 protein)。
- 若研究较新或小众，可扩展至DHRS蛋白家族其他成员的研究作为参考。
The recombinant human DHRS13 protein belongs to the short-chain dehydrogenase/reductase (SDR) superfamily, a group of enzymes conserved across species and involved in oxidation-reduction reactions, metabolism, and cellular detoxification. DHRS13 is encoded by the DHRS13 gene located on chromosome 17q12 and shares structural features typical of SDRs, including a conserved Rossmann-fold motif for NAD(P)+ cofactor binding and a catalytic tetrad critical for substrate specificity. While its precise physiological roles remain understudied, DHRS13 is hypothesized to participate in lipid and retinoid metabolism, potentially regulating retinoic acid levels, which influence cell differentiation, apoptosis, and immune responses. Dysregulation of SDR enzymes is often linked to metabolic disorders, cancer, and neurodegenerative diseases, implicating DHRS13 as a potential therapeutic target. Recombinant DHRS13 is produced via heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to enable functional and structural studies. Its recombinant form allows characterization of enzymatic activity, substrate identification, and interaction profiling. Current research focuses on elucidating its catalytic mechanism, tissue-specific expression, and involvement in pathways like steroidogenesis or inflammation. Challenges include clarifying post-translational modifications in recombinant forms and validating in vivo relevance. Further studies are needed to explore its diagnostic or therapeutic potential in human diseases.
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