| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX7C |
| Uniprot No | P15954 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-63aa |
| 氨基酸序列 | MLGQSIRRFTTSVVRRSHYEEGPGKNLPFSVENKWSLLAKMCLYFGSAFATPFLVVRHQLLKT |
| 分子量 | 32.56 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COX7C蛋白的模拟参考文献示例，涵盖功能、结构和疾病关联研究方向：
---
1. **文献名称**：**Recombinant Human COX7C Expression and Its Role in Mitochondrial Complex IV Assembly**
**作者**：Zhang L, et al.
**摘要**：本研究通过在大肠杆菌中表达重组人COX7C蛋白，探讨其在细胞色素c氧化酶(COX)复合物IV组装中的作用。实验表明，COX7C对复合物的稳定性至关重要，其缺失导致线粒体呼吸链功能异常。
2. **文献名称**：**Structural Characterization of Recombinant COX7C and Its Interaction with COX Supercomplexes**
**作者**：Wang Y, et al.
**摘要**：利用重组表达的COX7C蛋白进行X射线晶体学分析，揭示了其三维结构及其与COX复合物其他亚基的结合位点，为理解线粒体能量代谢调控机制提供了结构基础。
3. **文献名称**：**COX7C Knockdown via shRNA and Rescue with Recombinant Protein in Cancer Cell Metabolism**
**作者**：Kim S, et al.
**摘要**：通过构建COX7C敲低的癌细胞模型，并补充重组COX7C蛋白，证明其在维持癌细胞线粒体氧化磷酸化和增殖中的关键作用，提示其作为潜在抗癌靶点。
4. **文献名称**：**Mutation Analysis of Recombinant COX7C in Mitochondrial Encephalomyopathy**
**作者**：García-Castro M, et al.
**摘要**：在患者中发现COX7C基因突变后，重组表达突变体蛋白，证实其导致复合物IV活性下降，揭示了部分线粒体脑肌病的分子机制。
---
**说明**：以上文献为模拟内容，实际研究中请通过数据库(如PubMed、Web of Science)检索具体论文。若需真实文献，可结合关键词“recombinant human COX7C”“Cytochrome c oxidase subunit 7C”进一步筛选。
Cytochrome c oxidase subunit VIIc (COX7C) is a nuclear-encoded subunit of cytochrome c oxidase (Complex IV), the terminal enzyme in the mitochondrial electron transport chain (ETC). As part of the heme-copper oxidase family, Complex IV catalyzes the transfer of electrons from cytochrome c to molecular oxygen, driving proton translocation across the mitochondrial inner membrane and contributing to the proton gradient essential for ATP synthesis. Human COX7C, located on chromosome 5q14. is a small, highly hydrophobic protein (typically ~6.5 kDa) that plays a structural role in stabilizing the Complex IV holoenzyme and optimizing its catalytic activity.
Recombinant human COX7C protein is produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, enabling studies of its molecular interactions, regulatory functions, and role in mitochondrial energy metabolism. Dysregulation of COX7C has been linked to mitochondrial disorders, metabolic syndromes, and cancers, as impaired Complex IV assembly or function disrupts cellular bioenergetics and redox balance. Recombinant variants are also utilized to explore post-translational modifications, model pathogenic mutations, or develop targeted therapies for mitochondrial dysfunction. Research on COX7C contributes to understanding tissue-specific ETC adaptations and diseases associated with oxidative phosphorylation defects.
×
