| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX6B2 |
| Uniprot No | Q6YFQ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-88aa |
| 氨基酸序列 | MLDVEAQEPPKGKWSTPPFDPRFPSQNQIRNCYQNFLDYHRCLKTRTRRGKSTQPCEYYFRVYHSLCPISWVESWNEQIKNGIFAGKI |
| 分子量 | 36.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COX6B2蛋白的3篇参考文献示例(注：文献为模拟生成，实际引用时请核实真实来源)：
1. **"Expression and Functional Characterization of Recombinant Human COX6B2 in Mitochondrial Energy Metabolism"**
- **作者**: Zhang L, et al.
- **摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人COX6B2蛋白，证实其与细胞色素c氧化酶复合物的相互作用，并发现其在调节线粒体氧化磷酸化效率中的潜在作用。
2. **"COX6B2: A Testis-Specific Isoform Critical for Spermatogenesis"**
- **作者**: Tanaka K, et al.
- **摘要**: 通过基因敲除模型和重组COX6B2蛋白回补实验，揭示了COX6B2在雄性生殖细胞发育中的必要性，尤其在线粒体能量供应异常导致的精子活力缺陷中起关键作用。
3. **"Structural Insights into Human COX6B2 by Cryo-EM and Molecular Dynamics Simulation"**
- **作者**: Chen R, et al.
- **摘要**: 利用冷冻电镜和计算模拟解析了重组COX6B2的构象变化，提出了其在不同组织中线粒体复合物IV组装中的异构体特异性调节机制。
如需实际文献，建议通过PubMed或Web of Science检索关键词(如"COX6B2 recombinant"或"COX6B2 mitochondrial function")，并关注近五年相关研究。
Cytochrome c oxidase subunit 6B2 (COX6B2) is a nuclear-encoded subunit of mitochondrial complex IV (cytochrome c oxidase), a key component of the electron transport chain (ETC) critical for cellular energy production via oxidative phosphorylation. Unlike other COX subunits expressed ubiquitously, COX6B2 exhibits tissue-specific expression, predominantly in the testis, suggesting a specialized role in male reproductive tissues. It is thought to modulate complex IV assembly or activity under specific physiological conditions, though its exact function remains less characterized compared to other COX subunits.
Recombinant human COX6B2 protein is engineered through molecular cloning and heterologous expression systems (e.g., *E. coli* or mammalian cells) to facilitate functional studies. Its production enables research into COX6B2’s structural interactions within complex IV, regulatory mechanisms in energy metabolism, and potential links to mitochondrial disorders. Notably, COX6B2 dysfunction has been tentatively associated with oxidative stress and infertility, highlighting its relevance in reproductive biology and metabolic diseases.
Current applications include antibody development, protein-protein interaction assays, and exploring tissue-specific ETC adaptations. Further studies aim to clarify its role in spermatogenesis, hormonal regulation, and cellular responses to hypoxia, providing insights into targeted therapies for mitochondrial or fertility-related conditions.
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