| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX6A1 |
| Uniprot No | P12074 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-109aa |
| 氨基酸序列 | MAVVGVSSVSRLLGRSRPQLGRPMSSGAHGEEGSARMWKTLTFFVALPGVAVSMLNVYLKSHHGEHERPEFIAYPHLRIRTKPFPWGDGNHTLFHNPHVNPLPTGYEDE |
| 分子量 | 37.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COX6A1蛋白的参考文献示例(内容为假设性概括，仅供参考)：
1. **"Expression and Functional Analysis of Recombinant Human COX6A1 in Mitochondrial Complex IV Assembly"**
*Authors: Zhang Y. et al. (2020)*
**摘要**：本研究通过大肠杆菌表达系统成功制备重组人COX6A1蛋白，并验证其在体外促进线粒体复合体IV组装的功能，揭示其与神经退行性疾病相关的潜在分子机制。
2. **"Structural Characterization of COX6A1 and Its Role in Cancer Cell Metabolism"**
*Authors: Kim S. et al. (2018)*
**摘要**：通过X射线晶体学解析重组COX6A1蛋白的三维结构，结合基因敲除实验证明其在调控肿瘤细胞线粒体呼吸链活性及代谢重编程中的关键作用。
3. **"COX6A1 Recombinant Protein Ameliorates Ischemic Heart Injury in Mouse Models"**
*Authors: Chen L. et al. (2021)*
**摘要**：利用哺乳动物细胞系表达的重组COX6A1蛋白进行动物实验，发现其通过增强心肌细胞线粒体功能显著减轻缺血再灌注损伤，为心脏保护治疗提供新靶点。
4. **"Development of a COX6A1-Specific Antibody Using Recombinant Protein Antigen"**
*Authors: Müller R. et al. (2019)*
**摘要**：以重组COX6A1蛋白为抗原制备高特异性抗体，验证其在人组织样本中的表达差异，并探讨其作为线粒体功能障碍生物标志物的潜力。
(注：以上文献为模拟示例，实际研究中请通过PubMed或Web of Science等平台检索具体文献。)
Recombinant human COX6A1 (Cytochrome c oxidase subunit 6A1) is a nuclear-encoded subunit of cytochrome c oxidase (COX), the terminal enzyme in the mitochondrial respiratory chain. As part of Complex IV, COX6A1 plays a critical role in the electron transport chain by facilitating the transfer of electrons from cytochrome c to molecular oxygen, coupled with proton translocation across the mitochondrial inner membrane to support ATP synthesis. This 9.8 kDa protein is tightly associated with the catalytic core of COX, contributing to its structural stability and regulatory functions.
The recombinant form of COX6A1 is typically produced using bacterial or mammalian expression systems, enabling studies on its molecular interactions, structural dynamics, and role in oxidative phosphorylation. Researchers utilize recombinant COX6A1 to investigate mitochondrial disorders, as mutations or dysregulation of COX subunits are linked to metabolic diseases, neurodegenerative conditions, and cancer progression. Its involvement in cellular energy metabolism has also drawn interest in aging research and exercise physiology.
Recent advances in recombinant protein technology allow detailed characterization of COX6A1's post-translational modifications and interaction networks, providing insights into tissue-specific COX assembly. Studies employing recombinant COX6A1 have helped elucidate mechanisms underlying hypoxia adaptation and ROS (reactive oxygen species) modulation, highlighting its dual role in energy production and oxidative stress regulation.
×
