| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX7B |
| Uniprot No | P24311 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-80aa |
| 氨基酸序列 | MFPLVKSALNRLQVRSIQQTMARQSHQKRTPDFHDKYGNAVLASGATFCIVTWTYVATQVGIEWNLSPVGRVTPKEWRNQ |
| 分子量 | 34.43 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COX7B蛋白的3条参考文献，按简明格式整理：
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1. **文献名称**：*Expression and Functional Analysis of Recombinant Human COX7B in Mitochondrial Disease Models*
**作者**：Li Y. et al.
**摘要**：研究通过大肠杆菌系统成功表达重组人COX7B蛋白，验证其在线粒体复合体IV组装中的作用。实验表明，COX7B缺陷导致呼吸链功能异常，为相关疾病的机制提供了新见解。
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2. **文献名称**：*Structural Characterization of COX7B and Its Interaction with COX7A*
**作者**：Wang H. et al.
**摘要**：利用X射线晶体学解析了重组人COX7B的结构，揭示了其与COX7A亚基的关键结合位点，为线粒体呼吸链复合体的组装机制提供了分子基础。
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3. **文献名称**：*Role of Recombinant COX7B in Modulating Cellular Oxidative Stress Response*
**作者**：Zhang R. et al.
**摘要**：通过哺乳动物细胞表达重组COX7B蛋白，发现其过表达可降低活性氧(ROS)水平，增强细胞抗氧化能力，提示其在氧化应激相关疾病中的潜在治疗价值。
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**备注**：以上文献为虚拟示例，实际研究中建议通过**PubMed**或**Web of Science**以“COX7B”、“recombinant protein”、“mitochondrial complex IV”为关键词检索近期文献。
Cytochrome c oxidase subunit 7B (COX7B) is a nuclear-encoded structural component of cytochrome c oxidase (Complex IV), the terminal enzyme in the mitochondrial electron transport chain (ETC). As part of the heme-copper oxidase superfamily, Complex IV catalyzes the transfer of electrons from cytochrome c to molecular oxygen, driving proton translocation across the inner mitochondrial membrane and contributing to the proton gradient essential for ATP synthesis. COX7B, specifically, is a small hydrophobic subunit located in the matrix-facing side of Complex IV. Though its precise regulatory role remains under investigation, studies suggest it contributes to enzyme stability, optimal catalytic activity, and assembly of the multi-subunit complex.
The human COX7B gene, located on the X chromosome (Xq21.1), encodes a 80-amino-acid precursor protein processed into the mature 75-amino-acid form. Mutations in COX7B are linked to rare mitochondrial disorders, including infantile encephalopathy and microcephaly, highlighting its critical role in mitochondrial function. Recombinant human COX7B protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) to facilitate structural studies, functional assays, and investigations into mitochondrial pathologies. Its recombinant form enables researchers to explore COX7B’s interactions within Complex IV, dissect molecular mechanisms of disease-associated mutations, and develop targeted therapeutic strategies for ETC-related disorders.
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