| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX4NB |
| Uniprot No | O43402 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210aa |
| 氨基酸序列 | MPGVKLTTQAYCKMVLHGAKYPHCAVNGLLVAEKQKPRKEHLPLGGPGAHHTLFVDCIPLFHGTLALAPMLEVALTLIDSWCKDHSYVIAGYYQANERVKDASPNQVAEKVASRIAEGFSDTALIMVDNTKFTMDCVAPTIHVYEHHENRWRCRDPHHDYCEDWPEAQRISASLLDSRSYETLVDFDNHLDDIRNDWTNPEINKAVLHLC |
| 分子量 | 48.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COX4NB蛋白的模拟参考文献示例(仅供参考，具体文献需通过学术数据库核实)：
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1. **文献名称**：Recombinant Human COX4NB Expression in *E. coli*: Purification and Functional Characterization
**作者**：Smith A, et al.
**摘要**：研究报道了在大肠杆菌中高效表达重组人COX4NB蛋白的优化策略，并通过亲和层析纯化获得高纯度蛋白。功能实验表明，重组蛋白能够恢复COX缺陷型细胞系的线粒体呼吸功能，证实其生物活性。
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2. **文献名称**：Structural Insights into COX4NB’s Role in Mitochondrial Complex IV Assembly
**作者**：Zhang L, et al.
**摘要**：通过X射线晶体学解析重组人COX4NB蛋白的三维结构，并探讨其与线粒体呼吸链复合物IV其他亚基的相互作用，揭示其在线粒体氧化磷酸化中的关键调控作用。
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3. **文献名称**：COX4NB Overexpression Attenuates Hypoxia-Induced Apoptosis in Cardiomyocytes
**作者**：Wang Y, et al.
**摘要**：研究利用重组COX4NB蛋白处理缺氧心肌细胞，发现其通过抑制线粒体凋亡通路(如减少Caspase-3活化)保护细胞存活，提示其在缺血性心脏疾病中的潜在治疗价值。
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**建议**：以上内容为示例，实际文献请通过PubMed、Google Scholar等平台以“recombinant human COX4”或“COX4NB mitochondrial function”等关键词检索最新研究。若COX4NB为特定研究变体，请结合具体命名背景筛选文献。
Recombinant human COX4NB protein is a genetically engineered form of the nuclear-encoded subunit 4 of cytochrome c oxidase (COX), a critical component of the mitochondrial electron transport chain (ETC) involved in cellular energy production. COX, also known as Complex IV, catalyzes the final step of oxidative phosphorylation, transferring electrons to oxygen to form water while driving ATP synthesis. The COX4 subunit exists in two isoforms, COX4-1 and COX4-2. with COX4-1 being ubiquitously expressed under normoxia, while COX4-2 is hypoxia-inducible and stabilizes COX activity during low-oxygen conditions. The COX4NB variant may refer to a specific isoform or modified construct used for structural or functional studies.
Recombinant COX4NB is typically produced in bacterial or mammalian expression systems, often fused with affinity tags (e.g., His-tag) for purification. Its production enables detailed investigations into COX assembly, respiratory chain regulation, and mitochondrial dysfunction linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. Researchers utilize this protein to study isoform-specific roles in oxygen sensing, metabolic adaptation, and apoptosis. Additionally, it serves as a tool for developing therapeutics targeting mitochondrial disorders or modulating energy metabolism in pathological conditions.
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