| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX6B1 |
| Uniprot No | P14854 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-86aa |
| 氨基酸序列 | MAEDMETKIKNYKTAPFDSRFPNQNQTRNCWQNYLDFHRCQKAMTAKGGDISVCEWYQRVYQSLCPTSWVTDWDEQRAEGTFPGKI |
| 分子量 | 35.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的参考文献示例(非真实文献，供参考格式)：
1. **《重组人COX6B1蛋白的克隆表达及其在线粒体功能研究中的应用》**
- 作者：Zhang Y. et al.
- 摘要：本研究成功构建了人COX6B1基因的重组表达载体，通过大肠杆菌系统实现高效表达，并利用亲和层析纯化获得高纯度蛋白。功能实验表明该蛋白可恢复COX缺陷细胞的呼吸链活性。
2. **《COX6B1的结构解析及其与线粒体复合物IV的相互作用》**
- 作者：Smith J. et al.
- 摘要：通过X射线晶体学解析重组人COX6B1的三维结构，揭示其与细胞色素c氧化酶(复合物IV)其他亚基的结合位点，为呼吸链组装机制提供结构依据。
3. **《COX6B1基因突变与神经退行性疾病的关联研究》**
- 作者：Wang L. et al.
- 摘要：基于重组COX6B1蛋白的功能分析，发现特定突变体导致线粒体膜电位下降，提示其可能通过影响呼吸链功能参与帕金森病的病理过程。
4. **《重组COX6B1蛋白在肝癌细胞能量代谢调控中的作用》**
- 作者：Chen H. et al.
- 摘要：利用重组COX6B1过表达模型，证实其通过增强线粒体氧化磷酸化抑制肝癌细胞 Warburg效应，为靶向代谢的抗肿瘤策略提供依据。
---
建议通过PubMed、Web of Science或Google Scholar检索 **"recombinant human COX6B1"** 或 **"COX6B1 protein function"** 获取真实文献。
**Background of Recombinant Human COX6B1 Protein**
Cytochrome c oxidase subunit 6B1 (COX6B1) is a nuclear-encoded subunit of cytochrome c oxidase (COX), the terminal enzyme in the mitochondrial electron transport chain (ETC) responsible for catalyzing oxygen reduction and ATP synthesis. As part of Complex IV, COX6B1 contributes to the structural stability and functional regulation of the enzyme.
The human COX6B1 protein is a small, hydrophobic subunit (approximately 10 kDa) located in the mitochondrial inner membrane. It interacts with other COX subunits to maintain the enzyme’s integrity and optimize its activity under varying metabolic conditions. Dysregulation of COX6B1 has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer, emphasizing its role in cellular energy homeostasis.
Recombinant human COX6B1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional studies. Its recombinant form facilitates investigations into COX assembly, mitochondrial dysfunction mechanisms, and drug screening for metabolic or oxidative stress-related pathologies. Researchers also utilize it to explore tissue-specific isoforms or mutations associated with COX deficiency syndromes.
Overall, recombinant COX6B1 serves as a vital tool for dissecting mitochondrial biology and advancing therapeutic strategies targeting energy metabolism defects.
×
