| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKLFSF7 |
| Uniprot No | Q96FZ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-175aa |
| 氨基酸序列 | MSHGAGLVRTTCSSGSALGPGAGAAQPSASPLEGLLDLSYPRTHAALLKVAQMVTLLIAFICVRSSLWTNYSAYSYFEVVTICDLIMILAFYLVHLFRFYRVLTCISWPLSELLHYLIGTLLLLIASIVAASKSYNQSGLVAGAIFGFMATFLCMASIWLSYKISCVTQSTDAAV |
| 分子量 | 44.99 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CKLFSF7蛋白的3条参考文献示例(注：部分内容基于虚拟文献归纳)：
1. **文献名称**：Structural characterization and functional analysis of recombinant human CKLFSF7 protein
**作者**：Li X, et al.
**摘要**：本研究通过大肠杆菌系统成功表达纯化重组人CKLFSF7蛋白，解析其三维结构并发现其通过结合细胞表面趋化因子受体CCR3调控中性粒细胞迁移，揭示了其在炎症反应中的潜在作用。
2. **文献名称**：CKLFSF7 overexpression promotes tumor angiogenesis via PI3K/AKT pathway in hepatocellular carcinoma
**作者**：Wang Y, Zhang H, Zhou Q.
**摘要**：通过体外实验证实，重组人CKLFSF7蛋白通过激活PI3K/AKT信号通路增强血管内皮细胞增殖和管腔形成能力，提示其在肝癌微环境中的促血管生成机制。
3. **文献名称**：Development of a novel monoclonal antibody against human CKLFSF7 for diagnostic applications
**作者**：Guo R, et al.
**摘要**：研究团队制备了针对重组CKLFSF7蛋白的高特异性单克隆抗体，验证其在肺癌组织样本中的免疫组化检测效能，为癌症病理诊断提供新型生物标志物检测工具。
注：上述文献为示例性内容，实际研究中请以真实发表的学术论文为准。
Recombinant human CKLFSF7 (Chemokine-like factor superfamily member 7) is a protein encoded by the *CKLFSF7* gene, belonging to the CKLF-like MARVEL transmembrane domain-containing (CMTM) family. This family bridges chemokines and transmembrane-4 superfamily proteins, playing roles in immune regulation and cellular communication. CKLFSF7 is primarily expressed in immune-related tissues, including the spleen, thymus, and peripheral blood leukocytes, suggesting its involvement in immune responses. Structurally, it features a conserved MARVEL domain implicated in membrane trafficking and cell junction formation.
Studies indicate CKLFSF7 may regulate chemokine-mediated pathways, influencing leukocyte migration, inflammation, and tumor progression. Its dysregulation has been associated with cancers, such as hepatocellular carcinoma and glioblastoma, where it may modulate tumor microenvironment interactions or immune evasion. Recombinant CKLFSF7 protein, produced via prokaryotic or eukaryotic expression systems, enables functional studies to elucidate its precise mechanisms. Researchers use it to investigate ligand-receptor interactions, signaling cascades, and therapeutic targeting.
Despite its potential significance, CKLFSF7's detailed biological functions remain underexplored, necessitating further research to clarify its role in immunity and disease. This protein represents a promising candidate for understanding immune-oncology pathways and developing biomarker-based therapies.
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