| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CIZ1 |
| Uniprot No | Q9ULV3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-818aa |
| 氨基酸序列 | MFSQQQQQQLQQQQQQLQQLQQQQLQQQQLQQQQLLQLQQLLQQSPPQAPLPMAVSRGLPPQQPQQPLLNLQGTNSASLLNGSMLQRALLLQQLQGNLRGYGMASPGLAAPSLTPPQLATPNLQQFFPQATRQSLLGPPPVGVPMNPSQFNLSGRNPQKQARTSSSTTPNRKDSSSQTMPVEDKSDPPEGSEEAAEPRMDTPEDQDLPPCPEDIAKEKRTPAPEPEPCEASELPAKRLRSSEEPTEKEPPGQLQVKAQPQARMTVPKQTQTPDLLPEALEAQVLPRFQPRVLQVQAQVQSQTQPRIPSTDTQVQPKLQKQAQTQTSPEHLVLQQKQVQPQLQQEAEPQKQVQPQVHTQAQPSVQPQEHPPAQVSVQPPEQTHEQPHTQPQVSLLAPEQTPVVVHVCGLEMPPDAVEAGGGMEKTLPEPVGTQVSMEEIQNESACGLDVGECENRAREMPGVWGAGGSLKVTILQSSDSRAFSTVPLTPVPRPSDSVSSTPAATSTPSKQALQFFCYICKASCSSQQEFQDHMSEPQHQQRLGEIQHMSQACLLSLLPVPRDVLETEDEEPPPRRWCNTCQLYYMGDLIQHRRTQDHKIAKQSLRPFCTVCNRYFKTPRKFVEHVKSQGHKDKAKELKSLEKEIAGQDEDHFITVDAVGCFEGDEEEEEDDEDEEEIEVEEELCKQVRSRDISREEWKGSETYSPNTAYGVDFLVPVMGYICRICHKFYHSNSGAQLSHCKSLGHFENLQKYKAAKNPSPTTRPVSRRCAINARNALTALFTSSGRPPSQPNTQDKTPSKVTARPSQPPLPRRSTRLKT |
| 分子量 | 117.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and Purification of Recombinant Human CIZ1 Protein in Escherichia coli"**
*作者：Li Y, Zhang H, et al.*
**摘要**：研究报道了一种在大肠杆菌系统中高效表达和纯化重组人CIZ1蛋白的方法，通过优化诱导条件和亲和层析技术获得高纯度蛋白，为后续癌症生物标志物研究提供材料基础。
2. **"Structural Insights into CIZ1’s Role in DNA Replication Licensing"**
*作者：Jenkins TM, Rahman S, et al.*
**摘要**：通过X射线晶体学解析重组CIZ1蛋白结构，揭示其与细胞周期蛋白及DNA复制相关因子的相互作用机制，阐明其在DNA复制起始中的调控功能。
3. **"Recombinant CIZ1 as a Potential Serum Biomarker for Early Breast Cancer Detection"**
*作者：Cox R, et al.*
**摘要**：利用重组CIZ1蛋白制备抗体，开发ELISA检测法，发现乳腺癌患者血清中CIZ1水平显著升高，提示其在早期诊断中的潜在临床应用价值。
4. **"Functional Analysis of CIZ1 Splice Variants in Neurodegenerative Disorders"**
*作者：Wang L, et al.*
**摘要**：研究通过体外表达不同剪接变体的重组CIZ1蛋白，发现特定变体在神经元细胞核内异常聚集，可能参与阿尔茨海默病的病理进程。
注：以上为模拟文献摘要，实际引用请以真实文献为准。建议通过PubMed或Web of Science以“recombinant CIZ1 protein”为关键词搜索最新研究。
Cysteine-rich interacting zinc finger protein 1 (CIZ1) is a nuclear protein involved in DNA replication and cell cycle regulation. Initially identified as a CDKN1A (p21) binding partner, it plays a role in maintaining genomic stability by modulating cyclin-dependent kinase activity and anchoring replication machinery to the nuclear matrix. CIZ1 contains multiple zinc finger domains, a nuclear localization signal, and alternative splicing variants linked to tissue-specific functions. Its dysregulation has been associated with cancers, particularly due to overexpression in tumors, where it may act as a biomarker or therapeutic target.
Recombinant human CIZ1 protein is produced using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. This engineered protein enables in vitro studies of its interactions (e.g., with DNA, cyclins, or matrix proteins), structural analysis, and functional assays exploring its role in cell proliferation or differentiation. Notably, CIZ1's epigenetic regulatory functions in DNA methylation and chromatin remodeling have sparked interest in cancer and neurodegenerative disease research. Recombinant variants also facilitate drug screening and mechanistic studies, aiding the development of CIZ1-targeted therapies.
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