| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CISD2 |
| Uniprot No | Q8N5K1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-135aa |
| 氨基酸序列 | MVLESVARIVKVQLPAYLKRLPVPESITGFARLTVSEWLRLLPFLGVLALLGYLAVRPFLPKKKQQKDSLINLKIQKENPKVVNEINIEDLCLTKAAYCRCWRSKTFPACDGSHNKHNELTGDNVGPLILKKKEV |
| 分子量 | 41.7 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人CISD2蛋白的参考文献示例(注：部分为模拟虚构内容，实际文献需根据数据库核实)：
1. **标题**: "Recombinant Human CISD2 Protein Attenuates Mitochondrial Dysfunction in Age-Related Metabolic Disorders"
**作者**: Chen L., et al.
**摘要**: 本研究在大肠杆菌中表达并纯化了重组人CISD2蛋白，验证其通过维持线粒体膜稳定性及铁硫簇组装，改善衰老相关代谢紊乱模型中胰岛素抵抗的作用机制。
2. **标题**: "Structural Insights into the Iron-Sulfur Cluster Binding Properties of Human CISD2"
**作者**: Wang Y., Zhang R.
**摘要**: 通过X射线晶体学解析了重组人CISD2蛋白的3D结构，揭示了其保守的CDGSH结构域如何结合铁硫簇，为靶向CISD2的抗氧化治疗提供理论基础。
3. **标题**: "Overexpression of Recombinant CISD2 Enhances Autophagy and Reduces Apoptosis in Neurodegenerative Cell Models"
**作者**: Kim S., et al.
**摘要**: 在HEK293细胞中高效表达重组人CISD2蛋白，发现其通过调节Bcl-2/Bax通路抑制神经元凋亡，并促进自噬，提示其治疗阿尔茨海默病的潜力。
4. **标题**: "Optimized Purification Protocol for Functional Recombinant CISD2 Protein and Its Role in Cellular Iron Homeostasis"
**作者**: Müller F., et al.
**摘要**: 开发了一种基于镍柱亲和层析和尺寸排阻色谱的纯化方案，获得高纯度重组CISD2.体外实验证实其参与细胞铁稳态调控，与转铁蛋白受体相互作用。
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注：以上文献名称及内容为示例性质，实际文献需通过学术数据库(如PubMed、CNKI)检索确认。
The human CISD2 (CDGSH iron-sulfur domain-containing protein 2) gene encodes a mitochondrial outer membrane protein critical for maintaining mitochondrial integrity and cellular homeostasis. It belongs to the NEET protein family, characterized by a conserved CDGSH domain that binds iron-sulfur (Fe-S) clusters, which are essential for redox regulation and electron transport. CISD2 plays roles in regulating autophagy, apoptosis, and mitochondrial metabolism, with implications in aging and age-related diseases. Studies link CISD2 deficiency to accelerated aging, neurodegeneration (e.g., Alzheimer’s disease), and metabolic disorders, while its overexpression extends lifespan in animal models.
Recombinant human CISD2 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its purification often involves affinity tags to isolate the Fe-S cluster-bound form, which retains biological activity. Researchers use recombinant CISD2 to investigate its interaction with partners like BCL-2 or mitofusins, its role in calcium homeostasis, and its potential as a therapeutic target. Recent work explores its neuroprotective effects and modulation of oxidative stress pathways. As a regulator of mitochondrial dynamics and cellular energy balance, recombinant CISD2 remains a key tool in deciphering mechanisms underlying metabolic diseases, cancer, and degenerative disorders.
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