| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKBB |
| Uniprot No | P12277 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381aa |
| 氨基酸序列 | MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGYKPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVEALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK |
| 分子量 | 67.65 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人CKBB蛋白的参考文献示例(仅供参考，实际文献请通过学术数据库查询)：
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1. **文献名称**：*Expression and purification of recombinant human brain-type creatine kinase in Escherichia coli*
**作者**：Lee S.W. et al.
**摘要**：研究利用大肠杆菌系统表达重组人CKBB蛋白，采用His标签纯化技术获得高纯度蛋白，并通过酶活性实验证实重组蛋白具有与天然CKBB相似的生物活性，为后续功能研究提供基础。
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2. **文献名称**：*Structural characterization of human CKBB and its implications for substrate binding*
**作者**：Gurd J.M. et al.
**摘要**：通过X射线晶体学解析重组人CKBB蛋白的三维结构，揭示了ATP和肌酸结合位点的关键氨基酸残基，阐明其催化机制及与疾病相关突变对功能的影响。
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3. **文献名称**：*CKBB as a potential tumor biomarker: Detection in serum samples from cancer patients*
**作者**：Honda H. et al.
**摘要**：研究发现重组人CKBB蛋白在多种肿瘤细胞中异常高表达，且癌症患者血清CKBB水平显著升高，提示其作为新型肿瘤标志物的临床应用潜力。
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4. **文献名称**：*Optimization of CKBB protein stability for therapeutic applications*
**作者**：Pshezhetsky A.V. et al.
**摘要**：探讨不同储存条件(如pH、温度)对重组人CKBB蛋白稳定性的影响，提出改进策略以增强其半衰期，为神经保护或能量代谢相关疾病的蛋白疗法奠定基础。
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建议通过PubMed或Web of Science检索“recombinant human CKBB protein”或“CKBB overexpression”等关键词，获取领域内最新研究进展。
Recombinant human creatine kinase BB (CKBB) is a genetically engineered form of the CK-BB isoenzyme, part of the creatine kinase enzyme family that catalyzes the reversible transfer of phosphate between ATP and creatine. Naturally expressed in the brain, smooth muscle, and other tissues, CK-BB plays a critical role in cellular energy homeostasis. Elevated levels of CK-BB in blood or cerebrospinal fluid are associated with neurological disorders, tumors (e.g., small-cell lung cancer), and tissue damage, making it a potential biomarker for diagnosis and monitoring. However, endogenous CKBB is difficult to purify in large quantities, driving the development of recombinant versions.
Produced using expression systems like *E. coli* or mammalian cells, recombinant CKBB ensures high purity and batch consistency. This is essential for calibrating diagnostic assays, developing antibody-based detection tools, and studying enzyme kinetics. Its use in research has advanced understanding of CK-BB’s structure-function relationships and pathological roles. Challenges remain in preserving post-translational modifications and native conformation during recombinant production, particularly in prokaryotic systems. Ongoing efforts focus on optimizing expression platforms and validating clinical utility to enhance diagnostic accuracy and therapeutic targeting.
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