| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKLFSF3 |
| Uniprot No | P07335 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-182aa |
| 氨基酸序列 | MWPPDPDPDPDPEPAGGSRPGPAVPGLRALLPARAFLCSLKGRLLLAESGLSFITFICYVASSASAFLTAPLLEFLLALYFLFADAMQLNDKWQGLCWPMMDFLRCVTAALIYFAISITAIAKYSDGASKAAGVFGFFATIVFATDFYLIFNDVAKFLKQGDSADETTAHKTEEENSDSDSD |
| 分子量 | 45.76 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CKLFSF3蛋白的模拟参考文献示例(请注意，以下内容为虚构示例，仅供参考格式)：
1. **文献名称**: "Expression and Functional Analysis of Recombinant Human CKLFSF3 in Inflammatory Response"
**作者**: Zhang, Y. et al.
**摘要**: 研究报道了重组人CKLFSF3蛋白在大肠杆菌中的高效表达及纯化，通过体外实验证实其通过调控NF-κB信号通路抑制巨噬细胞的炎症因子释放，提示其在免疫调节中的潜在作用。
2. **文献名称**: "Structural Characterization of CKLFSF3 and Its Role in Cell Migration"
**作者**: Wang, L. & Chen, H.
**摘要**: 采用哺乳动物细胞系统表达重组CKLFSF3蛋白，通过晶体学解析其三维结构，发现其通过结合细胞表面受体CXCR4影响肿瘤细胞的迁移能力，为癌症治疗提供新靶点。
3. **文献名称**: "CKLFSF3 Recombinant Protein Enhances Epithelial Barrier Function in Intestinal Cells"
**作者**: Liu, J. et al.
**摘要**: 研究利用HEK293细胞表达带有His标签的重组CKLFSF3.证明其通过增强紧密连接蛋白ZO-1的表达改善肠上皮屏障完整性，可能在肠炎性疾病中具有保护作用。
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如需真实文献，建议通过PubMed或Web of Science搜索关键词"recombinant human CKLFSF3"获取。
**Background of Recombinant Human CKLFSF3 Protein**
The human CKLFSF3 (chemokine-like factor superfamily member 3) protein, encoded by the *CKLFSF3* gene, belongs to the chemokine-like factor (CKLF) family, which shares structural and functional similarities with chemokines. This protein is implicated in various cellular processes, including immune regulation, inflammation, and cell proliferation. CKLFSF3 is expressed in multiple tissues, with elevated levels observed in immune-related organs and certain cancers, suggesting roles in both physiological and pathological contexts.
Structurally, CKLFSF3 contains conserved motifs characteristic of chemokines, enabling interactions with cell surface receptors to modulate signaling pathways. Studies indicate its involvement in the NF-κB and MAPK pathways, influencing immune responses and tumor progression. However, its precise mechanisms remain partially unresolved, necessitating further exploration.
Recombinant human CKLFSF3 protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, ensuring post-translational modifications critical for bioactivity. This engineered protein serves as a vital tool for *in vitro* and *in vivo* studies, aiding in the characterization of its biological roles, receptor binding partners, and therapeutic potential. Current research focuses on its dual role in cancer—acting as either a tumor promoter or suppressor depending on context—and its interplay with immune cells in inflammatory diseases.
Overall, recombinant CKLFSF3 is pivotal for advancing insights into immune regulation and cancer biology, offering potential as a diagnostic marker or therapeutic target.
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