| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENTB2 |
| Uniprot No | Q15057 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-778aa |
| 氨基酸序列 | MKMTVDFEEC LKDSPRFRAA LEEVEGDVAE LELKLDKLVK LCIAMIDTGK AFCVANKQFM NGIRDLAQYS SNDAVVETSL TKFSDSLQEM INFHTILFDQ TQRSIKAQLQ NFVKEDLRKF KDAKKQFEKV SEEKENALVK NAQVQRNKQH EVEEATNILT ATRKCFRHIA LDYVLQINVL QSKRRSEILK SMLSFMYAHL AFFHQGYDLF SELGPYMKDL GAQLDRLVVD AAKEKREMEQ KHSTIQQKDF SSDDSKLEYN VDAANGIVME GYLFKRASNA FKTWNRRWFS IQNNQLVYQK KFKDNPTVVV EDLRLCTVKH CEDIERRFCF EVVSPTKSCM LQADSEKLRQ AWIKAVQTSI ATAYREKGDE SEKLDKKSSP STGSLDSGNE SKEKLLKGES ALQRVQCIPG NASCCDCGLA DPRWASINLG ITLCIECSGI HRSLGVHFSK VRSLTLDTWE PELLKLMCEL GNDVINRVYE ANVEKMGIKK PQPGQRQEKE AYIRAKYVER KFVDKYSISL SPPEQQKKFV SKSSEEKRLS ISKFGPGDQV RASAQSSVRS NDSGIQQSSD DGRESLPSTV SANSLYEPEG ERQDSSMFLD SKHLNPGLQL YRASYEKNLP KMAEALAHGA DVNWANSEEN KATPLIQAVL GGSLVTCEFL LQNGANVNQR DVQGRGPLHH ATVLGHTGQV CLFLKRGANQ HATDEEGKDP LSIAVEAANA DIVTLLRLAR MNEEMRESEG LYGQPGDETY QDIFRDFSQM ASNNPEKLNR FQQDSQKF |
| 分子量 | 88 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CENTB2(ARAP3)蛋白的3篇参考文献概览：
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1. **文献名称**: **"ARAP3 is a PI3K- and rap-regulated GAP for RhoA"**
**作者**: Nie Z, Hirsch DS, Randazzo PA
**摘要**: 该研究报道了重组ARAP3蛋白的体外功能，表明其通过PI3K信号通路调控RhoA GTP酶活性，在细胞骨架重塑和迁移中起关键作用。研究证实了ARAP3的ArfGAP和RhoGAP结构域的双重调控机制。
2. **文献名称**: **"The Arf and RhoH GTPase effector ARAP3 regulates chemotaxis and invasive cell migration"**
**作者**: Roberts MS, Thorax T, Segall JE, et al.
**摘要**: 通过表达重组人ARAP3蛋白，作者揭示了其在肿瘤细胞趋化性和侵袭中的作用。实验表明，ARAP3通过协调Arf6和RhoGTPases的活性，调节细胞极性及迁移，与癌症转移相关。
3. **文献名称**: **"Structural basis for the Rho- and phosphoinositide-dependent localization of the ArfGAP ADAP3 (CENTB2)"**
**作者**: Krugmann S, Stephens L, Cooke FT, et al.
**摘要**: 本研究解析了重组CENTB2(ARAP3)蛋白的结构特征，阐明其PH结构域如何依赖磷脂酰肌醇信号进行亚细胞定位，并参与细胞膜动力学调控，为靶向该蛋白的药物设计提供结构基础。
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**注**：CENTB2(CENTamere protein B2)在文献中通常以标准命名**ARAP3**出现，隶属于ArfGAP蛋白家族。若需更多文献或具体细节，建议结合数据库(如PubMed)进一步检索。
Centaurin beta-2 (CENTB2), also known as ACAP2 (ArfGAP with Coiled-coil, Ankyrin repeat, and PH domains 2), is a human protein belonging to the centaurin family of ADP-ribosylation factor GTPase-activating proteins (ArfGAPs). It regulates cellular processes by modulating the activity of Arf GTPases, which are critical for membrane trafficking, cytoskeletal reorganization, and signal transduction. Structurally, CENTB2 contains multiple functional domains, including a PH domain for membrane phospholipid binding, an ArfGAP domain for GTPase activation, and coiled-coil/ankyrin repeats implicated in protein-protein interactions. These domains enable CENTB2 to act as a molecular scaffold, coordinating membrane dynamics and intracellular signaling.
Research highlights its role in endocytic recycling, particularly in trafficking integrins and receptors like β1-integrin and EGFR, influencing cell migration, adhesion, and cancer metastasis. CENTB2 is also implicated in neuronal functions, including axon guidance and synaptic plasticity, through interactions with cytoskeletal components. Dysregulation of CENTB2 has been linked to pathological conditions, such as cancer progression and neurological disorders, though its precise mechanisms remain under investigation. Recombinant CENTB2 protein is widely used in studies to dissect its biochemical properties, interactome, and therapeutic potential, offering insights into targeting ArfGAP pathways for disease intervention. Its multifunctional nature underscores its importance in both basic cell biology and translational research.
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