| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPW |
| Uniprot No | Q5EE01 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-88aa |
| 氨基酸序列 | MALSTIVSQRKQIKRKAPRGFLKRVFKRKKPQLRLEKSGDLLVHLNCLLFVHRLAEESRTNACASKCRVINKEHVLAAAKVILKKSRG |
| 分子量 | 36.08 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人着丝粒蛋白W(CENPW)的参考文献示例，基于现有研究领域和常见研究方向整理：
1. **文献名称**：*CENPW Promotes Hepatocellular Carcinoma Progression Through Cell Cycle Regulation*
**作者**：Zhang Y, et al.
**摘要**：研究发现CENPW在肝细胞癌中显著过表达，通过调控细胞周期相关蛋白(如Cyclin B1)促进肿瘤细胞增殖，其高表达与患者不良预后相关。
2. **文献名称**：*CENPW Is Essential for Centromere Assembly and Chromosome Segregation in Mitosis*
**作者**：Li H, et al.
**摘要**：通过CRISPR干扰技术，揭示了CENPW在着丝粒蛋白复合体组装中的关键作用，并证明其缺失会导致染色体分离异常和细胞周期停滞。
3. **文献名称**：*CENPW as a Novel Biomarker in Triple-Negative Breast Cancer*
**作者**：Wang X, et al.
**摘要**：该研究提出CENPW在三阴性乳腺癌中的异常表达可作为潜在诊断标志物，并证实其通过激活PI3K/AKT通路促进肿瘤侵袭转移。
4. **文献名称**：*Structure and Functional Analysis of Recombinant Human CENPW Protein*
**作者**：Kimura T, et al.
**摘要**：首次报道了重组CENPW蛋白的体外表达与纯化方法，并通过X射线晶体学解析其结构，探讨其与其他着丝粒蛋白(如CENP-A)的相互作用机制。
**备注**：上述内容为领域内典型研究方向模拟，实际文献需通过PubMed、Web of Science或Google Scholar等平台检索确认。建议结合关键词“CENPW” + “cancer”/“centromere”/“cell cycle”等查询最新研究。
CENPW (Centromere Protein W) is a crucial component of the constitutive centromere-associated network (CCAN) in humans, playing a vital role in maintaining centromere integrity and ensuring accurate chromosome segregation during mitosis and meiosis. Discovered in the early 2000s, it is a small, evolutionarily conserved protein (∼20 kDa) characterized by its α-helical structure. CENPW interacts with other CCAN proteins, particularly CENP-T, to form the CENP-T-W-S-X complex, which anchors the kinetochore to centromeric DNA. This interaction facilitates the assembly of microtubule-binding kinetochore components, essential for proper spindle attachment and chromosome alignment.
Recombinant CENPW, produced via bacterial or eukaryotic expression systems, retains its native binding properties and is widely used in structural and functional studies. Its overexpression or dysregulation has been linked to genomic instability, aneuploidy, and cancers such as hepatocellular carcinoma and breast cancer. Notably, CENPW exhibits elevated expression in germ cells, suggesting specialized roles in gametogenesis. Current research focuses on its regulatory mechanisms, post-translational modifications (e.g., phosphorylation), and therapeutic targeting potential. Its dynamic expression during the cell cycle (peaking at G2/M phase) and tissue-specific roles underscore its importance in both basic cell biology and disease pathology.
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