| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENTB1 |
| Uniprot No | Q15027 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-740aa |
| 氨基酸序列 | MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL |
| 分子量 | 107.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CENTB1(ACAP1)蛋白的3-4篇参考文献的简单列举：
1. **文献名称**: "ACAP1 promotes cell migration by regulating Arf6 GTPase activity"
**作者**: Dai J. et al.
**摘要**: 该研究通过表达重组CENTB1蛋白，揭示了其通过激活Arf6的GTP酶活性调控细胞迁移的分子机制，并验证了其在细胞骨架重塑中的作用。
2. **文献名称**: "Structural basis of the PH domain and coiled-coil organization in ACAP1"
**作者**: Jackson T.R. et al.
**摘要**: 通过重组CENTB1蛋白的结晶分析，解析了其PH结构域和卷曲螺旋区域的三维结构，探讨了这些结构域在膜运输中的功能关联。
3. **文献名称**: "CENTB1 interacts with EGFR to regulate endocytic recycling and cancer progression"
**作者**: Saito K. et al.
**摘要**: 研究利用重组CENTB1蛋白进行体外结合实验，发现其与表皮生长因子受体(EGFR)互作，影响受体内吞循环并促进肿瘤细胞侵袭。
4. **文献名称**: "ACAP1 negatively modulates TLR4 signaling in macrophages"
**作者**: Nie Z. et al.
**摘要**: 通过重组蛋白功能实验，发现CENTB1通过抑制TLR4信号通路的过度激活，在巨噬细胞炎症反应中起负调控作用。
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**说明**：CENTB1(ACAP1)的功能研究多集中于Arf6相关的膜运输和疾病机制领域，以上文献涵盖结构解析、细胞迁移、肿瘤和免疫调控方向。如需具体应用方法(如重组蛋白表达系统)，可进一步筛选实验细节。
Centrosomal Protein 1B (CENTB1), also known as ARAP1 (ArfGAP with RhoGAP domain, Ankyrin repeat, and PH domain 1), is a multidomain regulatory protein involved in cellular signaling and cytoskeletal dynamics. It belongs to the ArfGAP family and functions as a GTPase-activating protein (GAP) for both ADP-ribosylation factor (Arf) and Rho GTPases, which are critical regulators of membrane trafficking, actin remodeling, and cell motility. CENTB1 contains several functional domains, including ArfGAP, RhoGAP, ankyrin repeats, and pleckstrin homology (PH) domains, enabling it to integrate signals from lipid second messengers like phosphatidylinositol (3.4.5)-trisphosphate [PI(3.4.5)P3] and coordinate cross-talk between Arf and Rho signaling pathways.
Recombinant human CENTB1 protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) and purified for in vitro studies. It is widely used to investigate its role in endocytosis, receptor recycling, and cell migration, particularly in cancer metastasis and immune cell regulation. Studies highlight its involvement in diseases such as glioblastoma, breast cancer, and neurodevelopmental disorders. By modulating CENTB1 activity, researchers aim to unravel its mechanistic contributions to pathological processes and explore potential therapeutic targets. Its recombinant form enables biochemical assays, structural studies, and screening for inhibitors or activators, making it a valuable tool in both basic and translational research.
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