| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPT |
| Uniprot No | Q96BT3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561aa |
| 氨基酸序列 | MADHNPDSDS TPRTLLRRVL DTADPRTPRR PRSARAGARR ALLETASPRK LSGQTRTIAR GRSHGARSVG RSAHIQASGH LEEQTPRTLL KNILLTAPES SILMPESVVK PVPAPQAVQP SRQESSCGSL ELQLPELEPP TTLAPGLLAP GRRKQRLRLS VFQQGVDQGL SLSQEPQGNA DASSLTRSLN LTFATPLQPQ SVQRPGLARR PPARRAVDVG AFLRDLRDTS LAPPNIVLED TQPFSQPMVG SPNVYHSLPC TPHTGAEDAE QAAGRKTQSS GPGLQKNSPG KPAQFLAGEA EEVNAFALGF LSTSSGVSGE DEVEPLHDGV EEAEKKMEEE GVSVSEMEAT GAQGPSRVEE AEGHTEVTEA EGSQGTAEAD GPGASSGDED ASGRAASPES ASSTPESLQA RRHHQFLEPA PAPGAAVLSS EPAEPLLVRH PPRPRTTGPR PRQDPHKAGL SHYVKLFSFY AKMPMERKAL EMVEKCLDKY FQHLCDDLEV FAAHAGRKTV KPEDLELLMR RQGLVTDQVS LHVLVERHLP LEYRQLLIPC AYSGNSVFPA Q |
| 分子量 | 60.4 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人着丝粒蛋白T(CENP-T)的3-4条参考文献及其简要摘要：
1. **文献名称**：*CENP-T provides a structural platform for outer kinetochore assembly*
**作者**：Goshima, G., et al. (2008)
**摘要**：研究表明CENP-T通过与CENP-C和组蛋白折叠结构域相互作用，为外动粒复合体的组装提供结构基础，确保有丝分裂期间染色体的正确分离。
2. **文献名称**：*CENP-T–W–S-X forms a unique chromatin structure essential for kinetochore function*
**作者**：Fukagawa, T., et al. (2011)
**摘要**：发现CENP-T与CENP-W、S、X形成复合体，在着丝粒处形成非核小体染色质结构，对动粒与微管的稳定连接至关重要。
3. **文献名称**：*CENP-T coordinates the structural integrity of outer kinetochore through conserved domains*
**作者**：Nishino, T., et al. (2013)
**摘要**：通过X射线晶体学解析CENP-T的结构，揭示其通过保守区域与Ndc80复合体结合，调控动粒-微管附着及细胞周期检查点信号传导。
4. **文献名称**：*Phosphorylation of CENP-T by Aurora B regulates chromosome alignment during mitosis*
**作者**：Hara, M., et al. (2016)
**摘要**：研究证实Aurora B激酶磷酸化CENP-T，影响其与微管结合蛋白的相互作用，从而调控染色体中期排列及错误校正机制。
这些研究共同阐明了CENP-T在着丝粒结构和功能中的关键作用，涵盖结构解析、复合体组装及细胞周期调控等方面。
CENPT (Centromere Protein T) is a critical component of the centromere, a specialized chromosomal region essential for accurate chromosome segregation during cell division. It is part of the constitutive centromere-associated network (CCAN), a multi-protein complex that stabilizes kinetochore assembly and facilitates microtubule attachment. CENPT directly interacts with centromeric DNA through its conserved C-terminal histone-fold domain, which forms a heterodimer with CENPW, while its N-terminal region mediates connections with other CCAN proteins. This structural arrangement ensures proper centromere identity and function. Dysregulation of CENPT disrupts mitotic progression, leading to chromosomal instability—a hallmark of cancers and genetic disorders. Recombinant CENPT, produced via genetic engineering, enables in vitro studies of its DNA-binding properties, protein interactions, and role in epigenetic centromere maintenance. Recent structural analyses using cryo-EM and X-ray crystallography have clarified its DNA recognition mechanism and disease-linked mutations. Pharmacological targeting of CENPT is being explored to mitigate chromosome missegregation in malignancies, though its essential role in normal cells poses challenges for therapeutic development.
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