| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPO |
| Uniprot No | Q9BU64 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-300aa |
| 氨基酸序列 | MEQANPLRPDGESKGGVLAHLERLETQVSRSRKQSEELQSVQAQEGALGTKIHKLRRLRDELRAVVRHRRASVKACIANVEPNQTVEINEQEALEEKLENVKAILQAYHFTGLSGKLTSRGVCVCISTAFEGNLLDSYFVDLVIQKPLRIHHHSVPVFIPLEEIAAKYLQTNIQHFLFSLCEYLNAYSGRKYQADRLQSDFAALLTGPLQRNPLCNLLSFTYKLDPGGQSFPFCARLLYKDLTATLPTDVTVTCQGVEVLSTSWEEQRASHETLFCTKPLHQVFASFTRKGEKLDMSLVS |
| 分子量 | 60.2 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人着丝粒蛋白O(CENPO)的3篇代表性文献的简要信息(基于研究领域经典及近期成果，虚构示例)：
1. **"CENPO regulates mitotic progression and serves as a potential biomarker in cancer"**
- **作者**: Shao, H., et al.
- **摘要**: 揭示CENPO在有丝分裂中通过调控纺锤体组装确保染色体正确分离，其表达异常与多种癌症(如乳腺癌、肺癌)患者预后不良相关，并验证CENPO敲低可抑制癌细胞增殖和迁移。
2. **"Functional analysis of CENPO in the mitotic checkpoint via RNAi screening"**
- **作者**: Fukagawa, T., et al.
- **摘要**: 利用RNA干扰技术沉默CENPO，发现其缺失导致染色体排列紊乱和纺锤体检查点功能受损，证明CENPO是连接动粒与微管的关键调控因子，参与细胞周期检验点信号传导。
3. **"CENPO overexpression links to genomic instability and therapeutic targeting in solid tumors"**
- **作者**: Nardi, F., et al.
- **摘要**: 分析临床样本发现CENPO在结肠癌和卵巢癌中显著过表达，高表达导致基因组不稳定性增加，并筛选出小分子抑制剂可选择性诱导CENPO高表达癌细胞凋亡，提示其作为治疗靶点的潜力。
如需具体文献，建议在PubMed或Google Scholar以“CENPO function”“CENPO cancer”等关键词检索真实论文。
CENPO (Centromere Protein O) is a key component of the constitutive centromere-associated network (CCAN), a multiprotein complex essential for centromere organization and kinetochore assembly during cell division. It localizes specifically to active centromeres, playing a critical role in maintaining genomic stability by ensuring accurate chromosome segregation in mitosis and meiosis. Discovered in the early 2000s, CENPO is part of the CENP-H/I/K complex within CCAN, which anchors the kinetochore to centromeric chromatin. This interaction facilitates microtubule attachment and spindle checkpoint signaling, preventing aneuploidy—a hallmark of cancer and developmental disorders.
Structurally, CENPO contains conserved motifs that mediate its incorporation into the centromere and interactions with other CCAN subunits. Studies show its depletion leads to severe mitotic defects, including misaligned chromosomes and delayed cell cycle progression. Notably, CENPO expression correlates with certain cancers, suggesting potential diagnostic or therapeutic relevance. Recombinant CENPO proteins, generated via molecular cloning, serve as tools to study centromere biology and mechanisms underlying chromosomal instability. Recent research also explores its epigenetic regulation and role in viral integration sites, expanding its biological significance beyond traditional mitotic functions. As an evolutionarily conserved protein, CENPO offers insights into the fundamental principles of chromosome inheritance across eukaryotes.
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