| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPC1 |
| Uniprot No | Q03188 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-542aa |
| 氨基酸序列 | MAASGLDHLKNGYRRRFCRPSRARDINTEQGQNVLEILQDCFEEKSLANDFSTNSTKSVPNSTRKIKDTCIQSPSKECQKSHPKSVPVSSKKKEASLQFVVEPSEATNRSVQAHEVHQKILATDVSSKNTPDSKKISSRNINDHHSEADEEFYLSVGSPSVLLDAKTSVSQNVIPSSAQKRETYTFENSVNMLPSSTEVSVKTKKRLNFDDKVMLKKIEIDNKVSDEEDKTSEGQERKPSGSSQNRIRDSEYEIQRQAKKSFSTLFLETVKRKSESSPIVRHAATAPPHSCPPDDTKLIEDEFIIDESDQSFASRSWITIPRKAGSLKQRTISPAESTALLQGRKSREKHHNILPKTLANDKHSHKPHPVETSQPSDKTVLDTSYALIGETVNNYRSTKYEMYSKNAEKPSRSKRTIKQKQRRKFMAKPAEEQLDVGQSKDENIHTSHITQDEFQRNSDRNMEEHEEMGNDCVSKKQMPPVGSKKSSTRKDKEESKKKRFSSESKNKLVPEEVTSTVTKSRRISRRPSDWWVVKSEESCLKC |
| 分子量 | 87.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人CENPC1蛋白的参考文献及其摘要概括：
1. **"The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres"**
- **作者**: Fukagawa, T., et al.
- **摘要**: 本文揭示了CENPC1与CENP-H/I复合物的相互作用，通过重组人CENPC1蛋白实验证明其在调节新CENP-A整合至着丝粒中的关键作用，并维持染色体稳定性。
2. **"Structural basis for recognition of centromere-specific histone CENP-A by the chaperone HJURP"**
- **作者**: Pentakota, S., et al.
- **摘要**: 利用重组人CENPC1蛋白结合冷冻电镜技术，解析了CENPC1与伴侣蛋白HJURP的结构互作机制，阐明其如何介导CENP-A在着丝粒的定向定位。
3. **"CENP-C directs a structural transition of CENP-A nucleosomes to maintain centromeric integrity"**
- **作者**: Carroll, C.W., et al.
- **摘要**: 研究发现重组人CENPC1蛋白通过诱导CENP-A核小体的构象变化，稳定着丝粒区域的染色质结构，确保有丝分裂中染色体正确分离。
4. **"Functional analysis of CENP-C mutations in human cancer cells reveals impaired kinetochore assembly"**
- **作者**: Toso, A., et al.
- **摘要**: 通过表达突变型重组CENPC1蛋白，发现癌症相关突变可破坏其与微管结合蛋白的相互作用，导致动粒组装异常和染色体不稳定性。
以上研究均围绕CENPC1在着丝粒功能、结构及疾病中的作用展开，利用重组蛋白技术推动机制解析。如需具体期刊信息或发表年份，可进一步补充关键词或通过PubMed/Google Scholar检索。
CENPC1 (Centromere Protein C1) is a critical component of the centromere, a specialized chromosomal region essential for accurate chromosome segregation during mitosis and meiosis. It plays a pivotal role in maintaining the structural integrity of the centromere and ensuring proper kinetochore assembly, which mediates attachment of spindle microtubules. Human CENPC1 is a conserved protein containing distinct N- and C-terminal domains. The N-terminal domain interacts with centromeric chromatin, including the histone H3 variant CENP-A, while the C-terminal region recruits other kinetochore proteins. Dysregulation or mutations in CENPC1 are linked to chromosomal instability, mitotic errors, and diseases such as cancer. Recombinant human CENPC1 protein, produced via expression systems like *E. coli* or mammalian cells, is widely used in structural studies, functional assays, and drug discovery to dissect centromere biology. Its recombinant form enables researchers to explore molecular mechanisms of centromere organization, kinetochore dynamics, and the impact of pathogenic variants. Studies on CENPC1 also contribute to understanding cell cycle regulation and developing therapeutic strategies targeting mitosis-related disorders.
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