| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDYL |
| Uniprot No | Q9Y232 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-598 |
| 氨基酸序列 | MTFQASHRSA WGKSRKKNWQ YEGPTQKLFL KRNNVSAPDG PSDPSISVSS EQSGAQQPPA LQVERIVDKR KNKKGKTEYL VRWKGYDSED DTWEPEQHLV NCEEYIHDFN RRHTEKQKES TLTRTNRTSP NNARKQISRS TNSNFSKTSP KALVIGKDHE SKNSQLFAAS QKFRKNTAPS LSSRKNMDLA KSGIKILVPK SPVKSRTAVD GFQSESPEKL DPVEQGQEDT VAPEVAAEKP VGALLGPGAE RARMGSRPRI HPLVPQVPGP VTAAMATGLA VNGKGTSPFM DALTANGTTN IQTSVTGVTA SKRKFIDDRR DQPFDKRLRF SVRQTESAYR YRDIVVRKQD GFTHILLSTK SSENNSLNPE VMREVQSALS TAAADDSKLV LLSAVGSVFC CGLDFIYFIR RLTDDRKRES TKMAEAIRNF VNTFIQFKKP IIVAVNGPAI GLGASILPLC DVVWANEKAW FQTPYTTFGQ SPDGCSTVMF PKIMGGASAN EMLLSGRKLT AQEACGKGLV SQVFWPGTFT QEVMVRIKEL ASCNPVVLEE SKALVRCNMK MELEQANERE CEVLKKIWGS AQGMDSMLKY LQRKIDEF |
| 分子量 | 66.4 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人染色域Y样蛋白(CDYL)的3篇代表性文献及其摘要概述：
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1. **"CDYL bridges REST and histone methyltransferases for gene repression and suppression of cellular transformation"**
- **作者**: Zhang et al. (2004)
- **摘要**: 发现CDYL作为转录抑制因子，通过与核受体共抑制因子(N-CoR)和组蛋白甲基转移酶(如G9a)相互作用，招募组蛋白H3K9甲基化修饰复合物，在基因沉默和抑制肿瘤转化中发挥关键作用。
2. **"The chromodomain protein CDYL is required for chromosome segregation and sperm development"**
- **作者**: Liu et al. (2017)
- **摘要**: 研究揭示CDYL在哺乳动物精子发生中的关键功能，证明其通过调控减数分裂过程中染色体的正确分离及组蛋白-鱼精蛋白转换，影响雄性生殖细胞发育和生育能力。
3. **"CDYL promotes the chemoresistance of small cell lung cancer by regulating the H3K27me3-mediated epigenetic silencing of CDKN1C"**
- **作者**: Jiang et al. (2020)
- **摘要**: 阐明CDYL通过增强Polycomb抑制复合物2(PRC2)介导的H3K27me3修饰，表观沉默抑癌基因CDKN1C，从而促进小细胞肺癌化疗耐药性的分子机制。
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以上文献覆盖了CDYL在表观遗传调控、生殖发育及肿瘤耐药性中的多重功能。如需更早期或特定方向的研究，可进一步补充。
CDYL (Chromodomain Y-like protein) is a chromatin-associated protein encoded by the *CDYL* gene in humans. It belongs to the chromodomain protein family, known for their roles in epigenetic regulation. Structurally, CDYL contains an N-terminal chromodomain that binds methylated histones (e.g., H3K9me2/3) and a C-terminal enoyl-CoA hydratase-like domain, though its enzymatic activity remains debated. CDYL primarily functions as a transcriptional corepressor, recruiting histone-modifying enzymes such as histone deacetylases (HDACs) and methyltransferases (HMTases) to compact chromatin and silence gene expression. It plays critical roles in gametogenesis, neurodevelopment, and cellular differentiation by regulating gene networks involved in these processes.
Notably, CDYL exists in two major isoforms: CDYL and CDYL2. CDYL is highly expressed in germ cells and testes, linking it to spermatogenesis and fertility. CDYL2. a shorter isoform, shows broader tissue expression and may have distinct regulatory functions. Dysregulation of CDYL has been implicated in various pathologies, including cancer. For example, CDYL acts as a tumor suppressor in prostate and liver cancers by repressing oncogenic pathways, but it may also promote tumor progression in other contexts by silencing differentiation genes. Its role in neuropsychiatric disorders, such as schizophrenia, is under investigation due to its impact on neuronal gene expression. Ongoing research aims to elucidate CDYL's precise molecular mechanisms and therapeutic potential in disease.
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