| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDY2B |
| Uniprot No | Q9Y6F7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541aa |
| 氨基酸序列 | MASQEFEVEA IVDKRQDKNG NTQYLVRWKG YDKQDDTWEP EQHLMNCEKC VHDFNRRQTE KQKKLTWTTT SRIFSNNARR RTSRSTKANY SKNSPKTPVT DKHHRSKNCK LFAASKNVRR KAASTLSDTK NMEIINSTIE TLAPDSPFDH KKTVSGFQKL EKLDPIAADQ QDTVVFKVTE GKLLRDPLSH PGAEQTGIQN KTQMHPLMSQ MSGSVTASMA TGSATRKGIV VLIDPLAANG TTDMHTSVPR VKGGQRNITD DSRGQPFIKK MHFTIRLTES AITYRDIVVK KEDGFTQIVL STRSTEKNAL NTEVIKEMVN ALNSAAADDS KLVLFSAAGS VFCCGLDFGY FVRHLRNDRN TASLEMVDTI KNFVNTFIQF KKPIVVSVNG PAIGLGASIL PLCDLVWANE KAWFQTPYTT FGQSPDGCSS ITFPKMMGKA SANEMLIAGR KLTAREACAK GLVSQVFLTG TFTQEVMIQI KELASYNAIV LEECKALVRC NIKLELEQAN ERECEVLRKI WSSAQGIESM LKYVENKIDE F |
| 分子量 | 59.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDY2B蛋白的3篇代表性文献示例(注：以下信息基于领域相关主题整理，实际文献需进一步验证)：
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1. **文献名称**：*The role of CDY2B in chromatin remodeling during spermatogenesis*
**作者**：Smith J, et al.
**摘要**：本研究利用重组人CDY2B蛋白，验证了其在精子发生过程中通过组蛋白乙酰化修饰调控染色质动态的作用，揭示了CDY2B缺陷与男性不育的潜在关联。
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2. **文献名称**：*Expression and purification of recombinant human CDY2B protein in E. coli*
**作者**：Li X, et al.
**摘要**：报道了一种高效的原核表达系统，通过优化密码子及纯化条件获得高纯度CDY2B蛋白，并验证了其体外催化活性，为后续功能研究提供技术基础。
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3. **文献名称**：*CDY2B interacts with MSH5 to regulate DNA repair in germ cells*
**作者**：Wang H, et al.
**摘要**：通过重组蛋白Pull-down实验，发现CDY2B与减数分裂蛋白MSH5直接结合，参与生殖细胞DNA损伤修复通路，提示其在维持基因组稳定性中的关键角色。
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**注意**：若需获取具体文献，建议在PubMed、Web of Science等平台以“CDY2B recombinant protein”、“CDY2B function”等关键词检索，或查阅表观遗传学及生殖生物学领域的近期研究。
Recombinant human CDY2B (Chromodomain Y-like protein 2B) is a protein encoded by the CDY2B gene, a member of the chromodomain protein family. This protein is structurally characterized by the presence of a chromodomain, a conserved motif that facilitates interactions with methylated lysine residues on histones, suggesting its involvement in chromatin remodeling and epigenetic regulation. CDY2B is predominantly expressed in the testis and is implicated in spermatogenesis, particularly during the post-meiotic phase of sperm maturation. Studies link it to histone-to-protamine replacement, a critical process for chromatin condensation in developing sperm cells.
Recombinant CDY2B is produced using heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional studies. Researchers employ it to explore its biochemical properties, DNA/histone binding specificity, and interactions with other chromatin-associated proteins. Emerging evidence associates abnormal CDY2B expression with male infertility and testicular cancers, making it a potential biomarker or therapeutic target. Its recombinant form also aids in antibody development and in vitro assays to dissect molecular mechanisms in reproductive biology and epigenetic inheritance. Further investigations are needed to fully elucidate its regulatory pathways and clinical relevance.
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