| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDY2 |
| Uniprot No | Q9Y6F7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541 |
| 氨基酸序列 | MASQEFEVEA IVDKRQDKNG NTQYLVRWKG YDKQDDTWEP EQHLMNCEKC VHDFNRRQTE KQKKLTWTTT SRIFSNNARR RTSRSTKANY SKNSPKTPVT DKHHRSKNCK LFAASKNVRR KAASTLSDTK NMEIINSTIE TLAPDSPFDH KKTVSGFQKL EKLDPIAADQ QDTVVFKVTE GKLLRDPLSH PGAEQTGIQN KTQMHPLMSQ MSGSVTASMA TGSATRKGIV VLIDPLAANG TTDMHTSVPR VKGGQRNITD DSRGQPFIKK MHFTIRLTES AITYRDIVVK KEDGFTQIVL STRSTEKNAL NTEVIKEMVN ALNSAAADDS KLVLFSAAGS VFCCGLDFGY FVRHLRNDRN TASLEMVDTI KNFVNTFIQF KKPIVVSVNG PAIGLGASIL PLCDLVWANE KAWFQTPYTT FGQSPDGCSS ITFPKMMGKA SANEMLIAGR KLTAREACAK GLVSQVFLTG TFTQEVMIQI KELASYNAIV LEECKALVRC NIKLELEQAN ERECEVLRKI WSSAQGIESM LKYVENKIDE F |
| 分子量 | 60.5 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDY2蛋白的参考文献示例(注：CDY2的研究相对较少，以下内容基于公开文献的可能方向进行合理模拟，部分信息可能需要核实)：
1. **文献名称**：**"Expression and Purification of Recombinant Human CDY2 Protein for Epigenetic Studies"**
**作者**：Smith A, et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效制备重组人CDY2蛋白的方法，并通过质谱和Western blot验证其结构与功能。研究表明，CDY2与组蛋白乙酰转移酶活性相关，可能在精子发生中参与染色质重塑。
2. **文献名称**：**"Functional Analysis of CDY2 in Chromatin Remodeling during Spermatogenesis"**
**作者**：Li X, et al.
**摘要**：通过重组CDY2蛋白的体外实验，发现其能与特定DNA序列结合并招募组蛋白修饰酶，促进染色质凝缩。实验表明CDY2缺失会导致小鼠精子发育异常，提示其在哺乳动物生育中的关键作用。
3. **文献名称**：**"Structural Insights into the Chromodomain of Human CDY2 and Its Interaction with Histones"**
**作者**：Wang Y, et al.
**摘要**：利用X射线晶体学解析了CDY2蛋白的染色质结构域(chromodomain)三维结构，发现其能特异性识别组蛋白H3K4me2/3修饰位点，为阐明其在表观遗传调控中的机制提供结构基础。
4. **文献名称**：**"CDY2 as a Potential Biomarker for Male Infertility: Role in Sperm Chromatin Compaction"**
**作者**：Gupta R, et al.
**摘要**：通过临床样本分析发现，重组CDY2蛋白表达水平与精子染色质完整性显著相关，提示其可能作为男性不育的分子标志物，并成为体外治疗靶点。
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**注意**：以上文献为模拟示例，实际研究可能需要查阅具体数据库(如PubMed、Web of Science)。若需实际文献，建议以“CDY2 protein recombinant”、“chromodomain Y-like 2 function”为关键词检索。
**Background of Recombinant Human CDY2 Protein**
Recombinant human CDY2 (Chromodomain Y-like 2) protein is a synthetic version of a naturally occurring protein encoded by the *CDY2* gene, which belongs to the chromodomain protein family. Chromodomains are evolutionarily conserved structural motifs known for their role in chromatin remodeling and epigenetic regulation, primarily through binding to methylated histones. CDY2 is predominantly expressed in the testes and is implicated in spermatogenesis, particularly during the maturation of male germ cells. Studies suggest its involvement in histone-to-protamine exchange, a critical process for sperm chromatin condensation, ensuring proper DNA packaging and genomic stability in developing spermatozoa.
The recombinant form of CDY2 is typically produced using bacterial or mammalian expression systems, enabling researchers to study its biochemical properties, interactions, and functional mechanisms *in vitro*. Its production facilitates investigations into male infertility disorders linked to aberrant chromatin remodeling or defective spermatogenesis. Additionally, CDY2’s potential role in epigenetic regulation extends to broader research areas, including cancer biology, where dysregulated chromatin modifiers contribute to disease progression. Recombinant CDY2 protein serves as a valuable tool for developing diagnostic assays, understanding reproductive biology, and exploring therapeutic strategies targeting epigenetic pathways. Further research aims to clarify its specific molecular targets and regulatory networks in both physiological and pathological contexts.
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