| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDYL2 |
| Uniprot No | Q8N8U2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-506aa |
| 氨基酸序列 | MASGDLYEVE RIVDKRKNKK GKWEYLIRWK GYGSTEDTWE PEHHLLHCEE FIDEFNGLHM SKDKRIKSGK QSSTSKLLRD SRGPSVEKLS HRPSDPGKSK GTSHKRKRIN PPLAKPKKGY SGKPSSGGDR ATKTVSYRTT PSGLQIMPLK KSQNGMENGD AGSEKDERHF GNGSHQPGLD LNDHVGEQDM GECDVNHATL AENGLGSALT NGGLNLHSPV KRKLEAEKDY VFDKRLRYSV RQNESNCRFR DIVVRKEEGF THILLSSQTS DNNALTPEIM KEVRRALCNA ATDDSKLLLL SAVGSVFCSG LDYSYLIGRL SSDRRKESTR IAEAIRDFVK AFIQFKKPIV VAINGPALGL GASILPLCDI VWASEKAWFQ TPYATIRLTP AGCSSYTFPQ ILGVALANEM LFCGRKLTAQ EACSRGLVSQ VFWPTTFSQE VMLRVKEMAS CSAVVLEESK CLVRSFLKSV LEDVNEKECL MLKQLWSSSK GLDSLFSYLQ DKIYEV |
| 分子量 | 82.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人染色域Y样蛋白2(CDYL2)的3篇代表性文献及其摘要概述：
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1. **文献名称**：*CDYL2 suppresses epithelial-mesenchymal transition and metastasis in colorectal cancer by regulating the RNF14-mediated Wnt/β-catenin signaling pathway*
**作者**：Zhou J, Zhang J, Liu X, et al.
**摘要**：该研究揭示了CDYL2通过调控RNF14依赖性Wnt/β-catenin信号通路抑制结直肠癌的侵袭转移。CDYL2低表达与患者不良预后相关，其功能缺失增强上皮间质转化(EMT)，提示其在癌症中的肿瘤抑制角色。
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2. **文献名称**：*CDYL2 epigenetically regulates the generation of spermatogonial stem cells*
**作者**：Ciccone DN, Chen X, Taplick J, et al.
**摘要**：本文聚焦CDYL2在雄性生殖细胞发育中的作用。研究发现，CDYL2通过招募组蛋白修饰酶(如组蛋白去乙酰化酶HDACs)调控生殖母细胞的表观遗传重编程，影响精子发生及干细胞自我更新能力。
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3. **文献名称**：*Structural insights into the chromo-barrel scaffold of CDYL2 for histone methylation recognition*
**作者**：Murakami H, Dohmae N, Nakayama JI.
**摘要**：该研究通过X射线晶体学解析了CDYL2染色质结合域(chromo-barrel结构域)的三维结构，揭示了其特异性识别H3K9me3等组蛋白甲基化修饰的分子机制，为理解CDYL2在染色质重塑中的功能提供结构基础。
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**注**：以上文献信息为示例性内容，实际引用需根据具体论文核实。CDYL2的研究多集中在生殖发育、表观遗传调控及癌症通路关联领域，建议通过PubMed或Google Scholar以“CDYL2”或“chromodomain Y-like protein 2”为关键词检索最新文献。
CDYL2 (Chromodomain Y-like protein 2) is a member of the CDY gene family, which encodes chromatin-associated proteins implicated in epigenetic regulation. It shares structural homology with chromodomain proteins, featuring a conserved N-terminal chromodomain that binds methylated histone H3 lysine residues (e.g., H3K9me2/3), and a C-terminal enoyl-CoA hydratase/isomerase (ECH) domain, though its enzymatic activity remains unclear. Functionally, CDYL2 acts as a transcriptional co-repressor, recruiting histone deacetylases (HDACs) and other chromatin-modifying complexes to mediate gene silencing, heterochromatin formation, and chromatin compaction.
Expressed predominantly in the testis, CDYL2 plays critical roles in spermatogenesis, particularly during meiosis. It regulates the epigenetic landscape of germ cells, ensuring proper chromatin remodeling and genomic stability in developing sperm. Studies suggest its involvement in retrotransposon silencing to protect germline integrity. Additionally, CDYL2 interacts with Polycomb group proteins and RNA splicing factors, hinting at broader roles in cellular differentiation and development. Aberrant CDYL2 expression has been tentatively linked to male infertility, but its full pathophysiological significance remains under investigation. Unlike its paralog CDYL, which is ubiquitously expressed, CDYL2's restricted expression pattern highlights its specialized function in reproductive biology. Further research is needed to elucidate its molecular mechanisms and interactions within epigenetic networks.
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