| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC45L |
| Uniprot No | O75419 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-566aa |
| 氨基酸序列 | MFVSDFRKEFYEVVQSQRVLLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELETAFLEHKEQFHYFILINCGANVDLLDILQPDEDTIFFVCDTHRPVNVVNVYNDTQIKLLIKQDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRLEEEIVEQTMRRRQRREWEARRRDILFDYEQYEYHGTSSAMVMFELAWMLSKDLNDMLWWAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS |
| 分子量 | 88 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDC45L蛋白的3篇示例性参考文献(格式为虚构，仅作结构示例，建议通过学术数据库检索真实文献)：
---
1. **文献名称**: *"CDC45L is essential for the assembly of the human DNA replication complex"*
**作者**: Zhang Y. et al.
**摘要**: 本文研究了重组人CDC45L蛋白在DNA复制起始复合物形成中的作用，通过体外重构实验证明CDC45L与MCM和GINS复合物协同作用，是复制叉前体组装的关键因子。
---
2. **文献名称**: *"Functional characterization of recombinant human CDC45L in cell cycle regulation"*
**作者**: Tanaka S. et al.
**摘要**: 作者利用杆状病毒系统表达重组CDC45L蛋白，发现其通过调控CDK2激酶活性影响S期进程，并揭示了其与Cdc7-Dbf4复合物的功能互作。
---
3. **文献名称**: *"Structural insights into CDC45L's role in cancer progression"*
**作者**: Wang X. et al.
**摘要**: 研究通过质谱和免疫共沉淀技术发现，重组CDC45L蛋白在多种癌细胞中异常高表达，并证实其通过与PCNA的相互作用促进肿瘤细胞的DNA复制和增殖。
---
**建议**：通过PubMed、Google Scholar等平台检索关键词(CDC45L、DNA replication initiation、recombinant protein)获取真实文献，重点关注《Nucleic Acids Research》《Cell Cycle》《Journal of Biological Chemistry》等期刊的近期研究。
Cell division control protein 45 homolog (CDC45L), also known as CDC45. is an essential regulator of DNA replication and cell cycle progression in eukaryotic cells. As a core component of the CMG helicase complex (comprising CDC45. MCM2-7. and GINS proteins), it plays a critical role in the initiation and elongation phases of DNA replication during the S phase. CDC45L facilitates the unwinding of double-stranded DNA, enabling replication fork progression and ensuring accurate genome duplication. Its recruitment to replication origins depends on cyclin-dependent kinase (CDK) and Dbf4-dependent kinase (DDK) activation, highlighting its tight regulation within cell cycle checkpoints.
Recombinant human CDC45L protein is produced using biotechnological platforms (e.g., bacterial or mammalian expression systems) for functional studies. Its purified form enables in vitro investigations into replication mechanisms, protein-protein interactions within the replication complex, and responses to replication stress. Aberrant CDC45L expression or mutations are implicated in genomic instability and carcinogenesis, making it a biomarker candidate in cancer research. Recent studies also explore its potential as a therapeutic target, particularly in malignancies with dysregulated DNA replication. Ongoing research utilizes recombinant CDC45L to screen small-molecule inhibitors and characterize structural motifs critical for helicase activity, advancing both basic science and translational applications.
×
