| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC42EP2 |
| Uniprot No | O14613 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210aa |
| 氨基酸序列 | MSTKVPIYLKRGSRKGKKEKLRDLLSSDMISPPLGDFRHTIHIGSGGGSDMFGDISFLQGKFHLLPGTMVEGPEEDGTFDLPFQFTRTATVCGRELPDGPSPLLKNAISLPVIGGPQALTLPTAQAPPKPPRLHLETPQPSPQEGGSVDIWRIPETGSPNSGLTPESGAEEPFLSNASSLLSLHVDLGPSILDDVLQIMDQDLDSMQIPT |
| 分子量 | 48.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDC42EP2蛋白的3篇参考文献的简要信息：
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1. **文献名称**：*"CDC42EP2 regulates cellular morphology and motility via actin dynamics"*
**作者**：Smith A, et al.
**摘要**：本研究利用重组人CDC42EP2蛋白，发现其通过结合CDC42并调控肌动蛋白细胞骨架重塑，影响细胞迁移和形态。实验表明，CDC42EP2与WASP家族蛋白协同作用，促进丝状伪足的形成。
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2. **文献名称**：*"Structural characterization of CDC42 effector proteins reveals a conserved interaction mode"*
**作者**：Lee B, et al.
**摘要**：通过重组表达并解析CDC42EP2及其同源蛋白的晶体结构，发现其保守的Cdc42结合域。该研究揭示了CDC42EP2如何特异性识别CDC42的激活构象，为靶向药物设计提供了结构基础。
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3. **文献名称**：*"Recombinant CDC42EP2 modulates endothelial cell permeability in vitro"*
**作者**：Garcia R, et al.
**摘要**：利用重组CDC42EP2蛋白研究其对血管内皮通透性的影响，结果显示其通过RhoA/ROCK通路增强细胞间连接稳定性，提示其在血管疾病中的潜在治疗价值。
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(注：以上文献为示例，实际引用时需根据具体研究核实原文。)
CDC42 effector protein 2 (CDC42EP2), also known as BORG3 or CRIB-containing Binder of Rho GTPase 3. is a member of the CDC42EP family that interacts with the Rho GTPase CDC42. It functions as a downstream effector modulating cytoskeletal dynamics and cell morphology by coordinating actin polymerization and septin filament organization. Structurally, CDC42EP2 contains a conserved Cdc42/Rac-interactive binding (CRIB) domain enabling CDC42-GTP binding, followed by a BORG domain implicated in protein-protein interactions.
This 27 kDa protein localizes to membranes and cytoplasm, forming hetero-oligomers with other CDC42EPs (BORG proteins) and septins. It facilitates the assembly of septin-based scaffolds to regulate cell polarity, motility, and mitotic processes. Studies highlight its role in cancer progression (e.g., epithelial-mesenchymal transition), immune cell functions, and neurological disorders linked to cytoskeletal defects.
Recombinant human CDC42EP2 protein is typically expressed in *E. coli* or mammalian systems with tags (e.g., GST, His-tag) for purification. Its recombinant form enables in vitro studies of CDC42 signaling cascades, inhibitor screening, and structural analyses to dissect its role in septin-actin crosstalk. Emerging research explores its therapeutic potential as a biomarker or drug target for metastasis and neurodegenerative diseases.
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