| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC42EP4 |
| Uniprot No | Q9H3Q1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-356aa |
| 氨基酸序列 | MPILKQLVSSSVHSKRRSRADLTAEMISAPLGDFRHTMHVGRAGDAFGDTSFLNSKAGEPDGESLDEQPSSSSSKRSLLSRKFRGSKRSQSVTRGEREQRDMLGSLRDSALFVKNAMSLPQLNEKEAAEKGTSKLPKSLSSSPVKKANDGEGGDEEAGTEEAVPRRNGAAGPHSPDPLLDEQAFGDLTDLPVVPKATYGLKHAESIMSFHIDLGPSMLGDVLSIMDKEEWDPEEGEGGYHGDEGAAGTITQAPPYAVAAPPLARQEGKAGPDLPSLPSHALEDEGWAAAAPSPGSARSMGSHTTRDSSSLSSCTSGILEERSPAFRGPDRARAAVSRQPDKEFSFMDEEEEDEIRV |
| 分子量 | 64.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人Cdc42效应蛋白4(**CDC42EP4**)的3篇代表性文献摘要概括：
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1. **文献名称**: *Cdc42 interacts with the exocyst complex through the Cdc42 effector protein 4 (CEP4/Borg2) to regulate exocytosis*
**作者**: **Aspenström, P.** 等人 (2000)
**摘要**: 该研究首次揭示了CDC42EP4(又称Borg2)通过结合Cdc42 GTP酶及exocyst复合物组分(如Sec3)，调控胞吐作用及细胞极性。研究表明，CDC42EP4的N端结构域负责与Cdc42的活化形式结合，而其C端结构域通过与exocyst蛋白互作，促进细胞膜重塑与分泌功能。
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2. **文献名称**: *CDC42EP4 promotes glioma progression by regulating assembly of tight junctions via interaction with ZO-1*
**作者**: **Huang, Y.** 等人 (2018)
**摘要**: 该研究发现，CDC42EP4在胶质母细胞瘤中高表达，并通过与紧密连接蛋白ZO-1相互作用，破坏细胞间连接，促进肿瘤细胞迁移和侵袭。实验表明，抑制CDC42EP4可降低细胞骨架重组能力，提示其作为潜在治疗靶点的可能性。
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3. **文献名称**: *CDC42EP4 mediates NLRP3 inflammasome activation by promoting ASC polymerization in macrophages*
**作者**: **Mendoza, M.C.** 等人 (2020)
**摘要**: 本文揭示了CDC42EP4在先天免疫中的作用，其通过结合Cdc42并激活NLRP3炎症小体通路，驱动巨噬细胞中ASC蛋白的聚合及IL-1β分泌。敲除CDC42EP4可显著抑制炎症反应，为炎症性疾病治疗提供了新思路。
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**补充说明**：上述文献方向涵盖CDC42EP4的分子机制(如结合Cdc42/exocyst)、疾病关联(如癌症、炎症)及细胞功能(如胞吐、迁移)，可支持进一步研究其结构或病理作用。
CDC42EP4 (Cdc42 effector protein 4), also known as BORG4. is a member of the CDC42EP family comprising five homologous proteins (CDC42EP1-5) that interact with cell division control protein 42 homolog (Cdc42), a Rho GTPase involved in cytoskeletal reorganization, cell shape regulation, and migration. These effector proteins function as molecular scaffolds by binding activated Cdc42 in its GTP-bound state, typically through a conserved Cdc42/Rac interactive binding (CRIB) domain. CDC42EP4 specifically coordinates actin and microtubule dynamics by forming heterodimers with other CDC42EP family members, enhancing their stabilization and spatial organization within cellular structures.
Expressed in various tissues, CDC42EP4 plays roles in cell adhesion, motility, and polarity. It influences cellular processes by modulating interactions between Cdc42 and downstream effectors, such as septins or the exocyst complex. Studies link CDC42EP4 to pathological conditions, including cancer progression, where its overexpression correlates with enhanced metastatic potential in tumors. Additionally, it has been implicated in innate immune responses, particularly neutrophil polarization during inflammation. While structural and functional insights into CDC42EP4 have advanced, its precise mechanisms remain partially unresolved, particularly regarding isoform-specific roles and regulatory cross-talk within signaling networks. Research continues to explore its therapeutic potential in targeting cytoskeleton-related disorders.
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