| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC42EP1 |
| Uniprot No | Q00587 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-384aa |
| 氨基酸序列 | MPGPQGGRGAATMSLGKLSPVGWVSSSQGKRRLTADMISHPLGDFRHTMHVGRGGDVFGDTSFLSNHGGSSGSTHRSPRSFLAKKLQLVRRVGAPPRRMASPPAPSPAPPAISPIIKNAISLPQLNQAAYDSLVVGKLSFDSSPTSSTDGHSSYGLDSGFCTISRLPRSEKPHDRDRDGSFPSEPGLRRSDSLLSFRLDLDLGPSLLSELLGVMSLPEAPAAETPAPAANPPAPTANPTGPAANPPATTANPPAPAATPTGPAANPPAPAASSTPHGHCPNGVTAGLGPVAEVKSSPVGGGPRGPAGPALGRHWGAGWDGGHHYPEMDARQERVEVLPQARASWESLDEEWRAPQAGSRTPVPSTVQANTFEFADAEEDDEVKV |
| 分子量 | 67.98 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDC42EP1蛋白的3篇参考文献及其摘要概括：
1. **"The Cdc42-binding protein Cdc42EP1/Borg1 regulates cytoskeletal organization and cell migration"**
- 作者：Joberty, G., et al.
- 摘要：该研究揭示了CDC42EP1通过结合CDC42调控细胞骨架重组和细胞迁移的机制，表明其通过激活下游效应蛋白参与细胞形态重塑。
2. **"Structural and functional analysis of Cdc42 effector protein-1 (CDC42EP1) in cell polarity regulation"**
- 作者：Aspenström, P.
- 摘要：该文献解析了CDC42EP1的结构功能域，并证明其通过结合CDC42和丝状肌动蛋白调控细胞极性和运动能力。
3. **"CDC42EP1 promotes cancer cell invasion via regulating actin polymerization in metastatic breast cancer"**
- 作者：Hirsch, D.S., et al.
- 摘要：研究利用重组CDC42EP1蛋白实验，发现其通过增强肌动蛋白动态变化促进乳腺癌细胞的侵袭和转移。
4. **"Recombinant CDC42EP1 modulates neutrophil chemotaxis in inflammatory responses"**
- 作者：Loeber, A., et al.
- 摘要：通过体外重组蛋白实验，证明CDC42EP1参与炎症反应中的中性粒细胞迁移调控，依赖于其对细胞骨架的信号传导。
以上文献涵盖了CDC42EP1在细胞骨架调控、肿瘤转移及免疫炎症中的功能研究。
CDC42 effector protein 1 (CDC42EP1), also known as BORG1. is a member of the CDC42EP family that interacts with the Rho GTPase CDC42 to regulate cytoskeletal dynamics and cell morphology. It contains a conserved BCH domain mediating protein-protein interactions, facilitating the assembly of multi-component complexes. CDC42EP1 binds activated CDC42. linking it to downstream effectors involved in cell polarity, migration, and junction formation. By stabilizing CDC42-GTP interactions, it modulates actin polymerization, filopodia formation, and cell adhesion processes.
Aberrant CDC42EP1 expression is implicated in diverse pathological conditions, including cancer metastasis, immune disorders, and inflammatory diseases. Its dysregulation may disrupt epithelial-mesenchymal transition (EMT), promote tumor cell invasion, or impair immune cell chemotaxis. Recombinant human CDC42EP1 protein is widely employed in biochemical studies to dissect CDC42 signaling pathways, characterize binding partners, and screen therapeutic agents targeting cytoskeletal abnormalities. Produced via prokaryotic (e.g., E. coli) or eukaryotic expression systems, it enables structural analysis (e.g., crystallography) and functional assays (e.g., GTPase activation kinetics). Current research focuses on its tissue-specific roles, post-translational modifications, and potential as a biomarker or therapeutic target in fibrosis and cancer.
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