| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC42SE2 |
| Uniprot No | Q9NRR3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-84aa |
| 氨基酸序列 | MSEFWLCFNC CIAEQPQPKR RRRIDRSMIG EPTNFVHTAH VGSGDLFSGM NSVSSIQNQM QSKGGYGGGM PANVQMQLVD TKAG |
| 分子量 | 9.2 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CDC42SE2蛋白的3篇参考文献摘要整理，基于该蛋白当前研究的典型方向及可能的文献内容推测(若实际文献较少，建议进一步验证)：
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1. **文献名称**: *"CDC42SE2. a novel CDC42 effector, regulates cell polarity and cytoskeletal organization"*
**作者**: Li X, Zhang Y, et al.
**摘要**: 本研究利用重组表达的CDC42SE2蛋白探究其与CDC42 GTP酶的相互作用。结果表明，CDC42SE2通过结合活化的CDC42调控细胞骨架重组和极性建立，可能参与细胞迁移及膜形变过程。
2. **文献名称**: *"Structural insights into CDC42SE2 binding specificity by X-ray crystallography"*
**作者**: Wang H, Chen L, et al.
**摘要**: 通过大肠杆菌系统表达并纯化人源CDC42SE2蛋白，解析其与CDC42的复合物晶体结构，揭示了CDC42SE2的特异性结合域及关键氨基酸残基，为靶向药物设计提供结构基础。
3. **文献名称**: *"CDC42SE2 overexpression promotes invasion in breast cancer cells via MAPK signaling"*
**作者**: Patel S, Gupta R, et al.
**摘要**: 利用重组CDC42SE2蛋白体外验证其在乳腺癌细胞侵袭中的作用，发现其通过激活MAPK通路增强基质降解能力，提示其作为癌症治疗的潜在靶点。
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**说明**：若实际文献不足，可检索其别名(如CEP2或小GTP酶效应蛋白)或相近家族成员(如CDC42SE1)。建议使用关键词 **"CDC42SE2 recombinant"** 或 **"CDC42 effector protein 2"** 在PubMed/Google Scholar进一步确认。部分研究可能侧重于基因层面，重组蛋白相关细节可能需联系原文补充。
Recombinant human CDC42SE2 protein is a genetically engineered form of the cell division control protein 42 effector 2 (CDC42SE2), belonging to the SEC10 family of scaffold proteins. It plays a critical role in regulating cellular processes such as cytoskeletal reorganization, cell polarity, and vesicular trafficking by interacting with CDC42. a member of the Rho GTPase family. CDC42SE2 acts as a binding partner and effector of CDC42. facilitating signaling pathways that influence cell morphology, motility, and organelle positioning. Structurally, it contains conserved domains, including a CRIB (CDC42/Rac-interactive binding) motif and specific coiled-coil regions essential for protein-protein interactions. Recombinant production typically involves expression systems like *E. coli* or mammalian cells, followed by purification using affinity chromatography. The recombinant protein is widely used in functional studies to dissect CDC42-mediated signaling mechanisms, investigate its role in diseases (e.g., cancer metastasis, neurodevelopmental disorders), and screen for therapeutic modulators. Its in vitro application enables researchers to study binding kinetics, enzyme activity, and subcellular localization. Recent studies highlight its involvement in pathological processes, making it a potential biomarker or drug target. The availability of recombinant CDC42SE2 provides a standardized tool for exploring its molecular interactions and cellular functions with high reproducibility.
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