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Recombinant Human CDC40 Protein

  • 中文名: 重组人(CDC40)蛋白
  • 别    名: Cdc40; Cell division cycle 40; Cell division cycle 40 homolog; EH binding Protein 3; EH-binding Protein 3; Ehb3; hPRP17
货号: PA2000-6584
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点CDC40
Uniprot NoO60508
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-579aa
氨基酸序列MSAAIAALAASYGSGSGSESDSDSESSRCPLPAADSLMHLTKSPSSKPSLAVAVDSAPEVAVKEDLETGVHLDPAVKEVQYNPTYETMFAPEFGPENPFRTQQMAAPRNMLSGYAEPAHINDFMFEQQRRTFATYGYALDPSLDNHQVSAKYIGSVEEAEKNQGLTVFETGQKKTEKRKKFKENDASNIDGFLGPWAKYVDEKDVAKPSEEEQKELDEITAKRQKKGKQEEEKPGEEKTILHVKEMYDYQGRSYLHIPQDVGVNLRSTMPPEKCYLPKKQIHVWSGHTKGVSAVRLFPLSGHLLLSCSMDCKIKLWEVYGERRCLRTFIGHSKAVRDICFNTAGTQFLSAAYDRYLKLWDTETGQCISRFTNRKVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGAVNTIVFVDENRRFVSTSDDKSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGAQNRFRLNKKKIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHPHETSKVITCGWDGLIKLWD
分子量91.9 kDa
蛋白标签GST-tag at N-terminal
缓冲液0
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人CDC40蛋白的参考文献示例(注:以下为虚拟文献,供参考格式):

1. **标题**: "Cloning and Functional Analysis of Human CDC40 in Pre-mRNA Splicing"

**作者**: Zhang Y, et al.

**摘要**: 该研究成功克隆并在大肠杆菌中表达重组人CDC40蛋白,证实其通过与剪接体因子U2AF65相互作用调控pre-mRNA剪接,为CDC40的分子机制提供依据。

2. **标题**: "CDC40 Promotes Tumorigenesis via Alternative Splicing in Colorectal Cancer"

**作者**: Wang L, et al.

**摘要**: 利用重组CDC40蛋白进行体外功能实验,揭示其在结直肠癌中通过调控靶基因(如BIRC5)的可变剪接促进癌细胞增殖和转移。

3. **标题**: "Structural Characterization of Recombinant Human CDC40 by Cryo-EM"

**作者**: Li X, et al.

**摘要**: 通过冷冻电镜解析重组CDC40的三维结构,发现其N端结构域对结合剪接体核心组分SF3B1至关重要,阐明其维持剪接复合体稳定性的结构基础。

4. **标题**: "CDC40 Deficiency Disrupts Cell Cycle Progression via Impaired Splicing"

**作者**: Kim H, et al.

**摘要**: 研究发现重组CDC40蛋白的缺失导致细胞周期停滞于G2/M期,其功能依赖于对细胞周期相关mRNA(如PLK1)的正确剪接调控。

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*注:上述文献及内容均为模拟示例,实际研究中请通过数据库(如PubMed、Web of Science)检索真实文献。*


背景信息

The recombinant human CDC40 protein, also known as cell division cycle 40 or pre-mRNA processing factor 17 (PRP17), plays a critical role in pre-mRNA splicing and cell cycle regulation. As a component of the spliceosome, CDC40 is essential for catalyzing the removal of introns from precursor mRNA, ensuring proper gene expression. It interacts with other splicing factors like PRP16 and PRP18 during the second catalytic step of splicing, facilitating conformational changes required for spliceosome activation. Structurally, CDC40 contains conserved domains, including nuclear localization signals and protein interaction motifs, enabling its nuclear localization and functional assembly into splicing complexes.

Beyond splicing, CDC40 is implicated in cell cycle control, particularly at the G2/M transition, by regulating transcripts of mitotic regulators. Dysregulation of CDC40 is linked to genomic instability and diseases, with overexpression observed in cancers such as colorectal carcinoma and glioblastoma, where it correlates with poor prognosis. Recombinant CDC40 proteins are typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, allowing studies on its biochemical properties, interaction networks, and role in disease mechanisms. Recent research highlights its potential as a therapeutic target, particularly in splicing-deficient cancers. Functional studies using recombinant CDC40 have advanced understanding of spliceosome dynamics, offering insights into RNA processing errors and their implications in human pathologies.


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