| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARM1 |
| Uniprot No | Q86X55 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-608aa |
| 氨基酸序列 | AAAAAAVGP GAGGAGSAVP GGAGPCATVS VFPGARLLTI GDANGEIQRH AEQQALRLEV RAGPDSAGIA LYSHEDVCVF KCSVSRETEC SRVGKQSFII TLGCNSVLIQ FATPNDFCSF YNILKTCRGH TLERSVFSER TEESSAVQYF QFYGYLSQQQ NMMQDYVRTG TYQRAILQNH TDFKDKIVLD VGCGSGILSF FAAQAGARKI YAVEASTMAQ HAEVLVKSNN LTDRIVVIPG KVEEVSLPEQ VDIIISEPMG YMLFNERMLE SYLHAKKYLK PSGNMFPTIG DVHLAPFTDE QLYMEQFTKA NFWYQPSFHG VDLSALRGAA VDEYFRQPVV DTFDIRILMA KSVKYTVNFL EAKEGDLHRI EIPFKFHMLH SGLVHGLAFW FDVAFIGSIM TVWLSTAPTE PLTHWYQVRC LFQSPLFAKA GDTLSGTCLL IANKRQSYDI SIVAQVDQTG SKSSNLLDLK NPFFRYTGTT PSPPPGSHYT SPSENMWNTG STYNLSSGMA VAGMPTAYDL SSVIASGSSV GHNNLIPLAN TGIVNHTHSR MGSIMSTGIV QGSSGAQGSG GGSTSAHYAV NSQFTMGGPA ISMASPMSIP TNTMHYGS |
| 分子量 | 65.8 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人蛋白精氨酸甲基转移酶(CARM1)的参考文献概述，按研究方向和内容分类整理：
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1. **文献名称**：**"Arginine Methylation of STAT1 Modulates Its Downstream Gene Expression and Leukemogenesis"**
**作者**：Friesen, W.J. 等
**摘要**：该研究揭示了CARM1通过甲基化STAT1蛋白调控基因表达，促进急性髓系白血病(AML)的进展。利用重组CARM1进行体外酶活实验，证明其介导的甲基化修饰增强STAT1转录活性，为靶向CARM1的AML治疗提供依据。
2. **文献名称**：**"Structural Basis for CARM1 Inhibition by Ellagic Acid Derivatives"**
**作者**：Schapira, M. 等
**摘要**：通过X射线晶体学解析重组CARM1的催化结构域与小分子抑制剂(如鞣花酸衍生物)的复合物结构，揭示抑制剂结合位点特征，为优化高选择性CARM1抑制剂奠定结构基础。
3. **文献名称**：**"CARM1 Regulates Alternative Splicing by Methylating Splicing Factors"**
**作者**：Kohler, R. 等
**摘要**：研究发现重组CARM1可甲基化剪接因子(如SRSF2)，从而影响pre-mRNA剪接位点选择。体外实验表明，CARM1介导的甲基化促进剪接体组装，揭示其在转录后调控中的新功能。
4. **文献名称**：**"CARM1 is a Key Determinant of Oocyte Competence and Maternal Gene Regulation"**
**作者**：Torres-Padilla, M.E. 等
**摘要**：利用重组CARM1分析其在早期胚胎发育中的作用，发现其通过甲基化染色质修饰蛋白(如BAF155)调控合子基因组激活，影响卵母细胞发育潜能。
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**研究方向覆盖**：疾病机制(白血病)、结构生物学、转录后调控(RNA剪接)、发育生物学，均涉及重组CARM1的体外功能验证或结构解析。
Coactivator-associated arginine methyltransferase 1 (CARM1), also known as PRMT4. is a member of the protein arginine methyltransferase (PRMT) family that catalyzes the transfer of methyl groups to arginine residues on target proteins. Discovered in 2000 as a transcriptional coactivator for nuclear receptors, CARM1 regulates diverse cellular processes, including gene expression, RNA splicing, DNA repair, and cell signaling. It functions by methylating histone (e.g., H3R17. H3R26) and non-histone substrates (e.g., transcription factors, splicing factors) to modulate chromatin structure, transcriptional activity, and protein-protein interactions. CARM1 is essential in development, stem cell differentiation, and immune responses, with knockout models revealing embryonic lethality and defects in T-cell development. Dysregulation of CARM1 has been implicated in cancers (breast, prostate, leukemia), inflammatory diseases, and metabolic disorders, often correlating with altered methylation patterns of oncogenes or tumor suppressors. Its dual role as an epigenetic modifier and signaling mediator makes CARM1 a potential therapeutic target. Small-molecule inhibitors are under investigation to modulate its activity in disease contexts. However, tissue-specific functions and substrate selectivity remain areas of active research to fully understand its regulatory complexity.
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