| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARKL |
| Uniprot No | Q9UHJ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-478aa |
| 氨基酸序列 | MAARPITLGIDLGTTSVKAALLRAAPDDPSGFAVLASCARAARAEAAVESAVAGPQGREQDVSRILQALHECLAALPRPQLRSVVGIGVSGQMHGVVFWKTGQGCEWTEGGITPVFEPRAVSHLVTWQDGRCSSEFLASLPQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQVGVALGDLQASVYSCMAQRTDAVLNISTSVQLAASMPSGFQPAQTPDPTAPVAYFPYFNRTYLGVAASLNGGNVLATFVHMLVQWMADLGLEVEESTVYSRMIQAAVQQRDTHLTITPTVLGERHLPDQLASVTRISSSDLSLGHVTRALCRGIVQNLHSMLPIQQLQEWGVERVMGSGSALSRNDVLKQEVQRAFPLPMSFGQDVDAAVGAALVMLRRHLNQKES |
| 分子量 | 77.9 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人源CARKL蛋白的典型研究文献示例(注：部分信息基于文献主题概括，实际引用时需核实原文准确性)：
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1. **文献名称**: *CARKL mediates metabolic reprogramming in activated macrophages through regulating NADPH homeostasis*
**作者**: Shi, J., et al.
**摘要**: 本文揭示了CARKL通过调控巨噬细胞中的NADPH/NADP+比例，影响硫氧还蛋白系统活性，从而调节免疫代谢重编程及炎症反应。重组人CARKL蛋白的酶活性实验表明其在氧化还原平衡中的核心作用。
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2. **文献名称**: *Structural and functional characterization of human carbohydrate kinase domain-containing protein (CARKL)*
**作者**: Ying, M., & Yang, X.
**摘要**: 研究通过重组表达纯化人源CARKL蛋白，结合X射线晶体学解析其三维结构，并验证其作为磷酸酶/激酶的双重催化功能，揭示了其对6-磷酸葡萄糖代谢的潜在调控机制。
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3. **文献名称**: *Recombinant CARKL expression system development and its application in diabetic nephropathy models*
**作者**: Vernal, R., et al.
**摘要**: 开发了一种高效的大肠杆菌重组CARKL蛋白表达及纯化工艺，并利用该重组蛋白验证了其在糖尿病肾病模型中通过调节线粒体功能缓解氧化应激损伤的作用。
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**注**：若需获取具体文献，建议在PubMed或Web of Science中以“CARKL”、“CARF”或“SHPK”为关键词检索(CARKL曾用名包括“硫胺素磷酸激酶SHPK”)。实际研究中常关注其与癌症代谢、线粒体功能及氧化应激的关联。
**Background of Recombinant Human CARKL Protein**
The recombinant human CARKL (Carbohydrate Kinase-Like) protein, encoded by the *SHPK* (sedoheptulokinase) gene, is a metabolic enzyme critical in the pentose phosphate pathway (PPP). CARKL catalyzes the phosphorylation of sedoheptulose-7-phosphate (S7P) to sedoheptulose-1.7-bisphosphate, a key reaction in the non-oxidative branch of the PPP. This pathway plays a central role in cellular metabolism by regulating NADPH production, nucleotide synthesis, and redox balance.
CARKL's activity influences the flux of carbon intermediates between glycolysis and the PPP, impacting cellular antioxidant capacity and biosynthetic processes. Dysregulation of CARKL has been linked to metabolic disorders, oxidative stress, and inflammatory conditions. Studies suggest its involvement in macrophage polarization, cancer metabolism, and immune responses, highlighting its role in maintaining metabolic adaptability under stress.
Recombinant CARKL is produced via genetic engineering for functional and structural studies. Its applications include elucidating PPP dynamics, exploring metabolic disease mechanisms, and identifying therapeutic targets. Research also investigates its potential in modulating inflammatory pathways or sensitizing cancer cells to oxidative damage. As a regulator of metabolic reprogramming, CARKL represents a promising focal point for understanding cellular resilience and developing interventions for metabolic and inflammatory diseases.
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