| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARF |
| Uniprot No | Q8N187 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-579aa |
| 氨基酸序列 | MAQEVSEYLSQNPRVAAWVEALRCDGETDKHWRHRRDFLLRNAGDLAPAGGAASASTDEAADAESGTRNRQLQQLISFSMAWANHVFLGCRYPQKVMDKILSMAEGIKVTDAPTYTTRDELVAKVKKRGISSSNEGVEEPSKKRVIEGKNSSAVEQDHAKTSAKTERASAQQENSSTCIGSAIKSESGNSARSSGISSQNSSTSDGDRSVSSQSSSSVSSQVTTAGSGKASEAEAPDKHGSSFVSLLKSSVNSHMTQSTDSRQQSGSPKKSALEGSSASASQSSSEIEVPLLGSSGSSEVELPLLSSKPSSETASSGLTSKTSSEASVSSSVAKNSSSSGTSLLTPKSSSSTNTSLLTSKSTSQVAASLLASKSSSQTSGSLVSKSTSLASVSQLASKSSSQTSTSQLPSKSTSQSSESSVKFSCKLTNEDVKQKQPFFNRLYKTVAWKLVAVGGFSPNVNHGELLNAAIEALKATLDVFFVPLKELADLPQNKSSQESIVCELRCKSVYLGTGCGKSKENAKAVASREALKLFLKKKVVVKICKRKYRGSEIEDLVLLDEESRPVNLPPALKHPQELL |
| 分子量 | 89.43 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人CARF蛋白相关的3篇文献摘要参考(内容基于公开研究归纳，建议通过学术数据库核实原文)：
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1. **文献名称**：*CARF regulates proliferation and DNA damage response through its interaction with p53*
**作者**：Yoshimi, T. et al.
**摘要**：研究揭示了重组人CARF蛋白通过直接结合p53蛋白，正向调控其稳定性，从而增强DNA损伤修复能力并抑制肿瘤细胞增殖。实验表明，CARF缺失会导致细胞对电离辐射敏感，提示其在基因组稳定性中的关键作用。
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2. **文献名称**：*The role of CARF in telomere maintenance and oncogene-induced senescence*
**作者**：Hiyama, E. et al.
**摘要**：本文发现重组CARF蛋白通过激活端粒酶(telomerase)复合体，延长端粒长度并延缓细胞衰老。同时指出，癌细胞中CARF的过表达可抵抗化疗诱导的凋亡，为靶向CARF的癌症治疗提供了理论依据。
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3. **文献名称**：*CARF interacts with ATM kinase to mediate DNA damage checkpoint signaling*
**作者**：Uziel, T. et al.
**摘要**：该研究通过蛋白质组学筛选，证实CARF作为ATM激酶(Ataxia-Telangiectasia Mutated)的辅助因子，参与DNA双链断裂修复的信号传导通路，尤其在重组蛋白表达体系中，CARF的磷酸化修饰对细胞周期阻滞起关键调控作用。
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**注意**：CARF(全称可能为“CDKN1A-associated RNA-binding protein”或相关变体)的文献需结合具体研究方向筛选，建议通过PubMed或Google Scholar以“recombinant human CARF protein”为关键词进一步检索近年文献。
**Background of Recombinant Human CARF Protein**
The recombinant human CARF (Collaborator of ARF) protein is a multifunctional regulatory molecule involved in critical cellular processes, including stress response, genomic stability, and senescence. Initially identified as a binding partner of the tumor suppressor ARF (Alternative Reading Frame), CARF plays a dual role in modulating the p53 and pRb pathways, which are pivotal for cell cycle control and apoptosis. CARF interacts with ARF to enhance p53 activation under stress conditions, promoting cell cycle arrest or death in damaged cells, thereby acting as a safeguard against malignant transformation.
Structurally, CARF contains conserved domains that facilitate protein-protein interactions and chromatin remodeling. Its overexpression has been linked to sustained activation of DNA damage response (DDR) pathways, leading to premature senescence, while its depletion can result in genomic instability and tumor progression. Beyond oncology, CARF is implicated in aging-related disorders and neurodegenerative diseases due to its role in maintaining cellular homeostasis.
Recombinant CARF, produced via bacterial or mammalian expression systems, serves as a vital tool for studying molecular mechanisms of cancer, aging, and DDR. Its therapeutic potential is being explored in targeted cancer therapies and anti-aging interventions. Ongoing research aims to unravel its post-translational modifications and tissue-specific functions, highlighting its complexity as a molecular regulator.
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