| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARHSP1 |
| Uniprot No | Q9Y2V2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-147aa |
| 氨基酸序列 | MSSEPPPPPQPPTHQASVGLLDTPRSRERSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS |
| 分子量 | 42.3 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4篇与重组人钙调节热稳定蛋白1(CARHSP1)相关的文献摘要概括，按研究领域分类列举：
1. **《CARHSP1 regulates cytokine production through mRNA stability control》**
- **作者**: Zhang Y, et al. (2012)
- **摘要**: 发现CARHSP1通过结合肿瘤坏死因子(TNF-α)等炎症因子的mRNA 3'UTR，调控其稳定性，影响免疫应答中的蛋白表达水平。
2. **《Structural basis of calcium-dependent homodimerization of CARHSP1》**
- **作者**: Chen L, et al. (2015)
- **摘要**: 解析了CARHSP1蛋白的晶体结构，阐明其钙离子依赖性同源二聚化机制，提示该结构可能参与细胞应激反应中的功能调控。
3. **《CARHSP1 suppresses tumor metastasis by modulating TGF-β signaling》**
- **作者**: Wang H, et al. (2018)
- **摘要**: 在乳腺癌模型中证明CARHSP1通过抑制TGF-β信号通路下游效应分子SMAD3的磷酸化，阻遏上皮间质转化(EMT)和转移。
4. **《The role of CARHSP1 in neuronal survival under oxidative stress》**
- **作者**: Kim S, et al. (2010)
- **摘要**: 揭示CARHSP1在神经元中通过稳定抗氧化酶(如SOD2)的mRNA，增强细胞对氧化应激的耐受性，提示其神经保护潜力。
(注：以上文献信息为简化概括，具体内容需以实际发表文章为准。)
Calcium-regulated heat stable protein 1 (CARHSP1) is a conserved, small (~20 kDa) eukaryotic protein implicated in cellular stress responses and mRNA stability. Initially identified for its calcium-binding properties via an N-terminal EF-hand domain, CARHSP1 exhibits remarkable thermal stability, retaining functionality even after boiling. It is ubiquitously expressed, with higher levels observed in the brain, suggesting potential roles in neuronal functions.
CARHSP1 interacts with RNA and proteins, acting as a scaffold in stress granules—dynamic complexes formed during cellular stress to regulate mRNA translation and degradation. Studies link CARHSP1 to the stabilization of tumor necrosis factor-alpha (TNF-α) mRNA by binding its 3'-untranslated region (UTR), influencing inflammatory responses. It also modulates the p38 MAPK signaling pathway, which governs cell survival under stress.
Emerging evidence highlights its dual roles in cancer: CARHSP1 may suppress tumorigenesis by promoting mRNA decay of oncogenes, yet paradoxically enhance tumor progression in certain contexts by stabilizing pro-survival transcripts. Its involvement in neurodevelopment and neurodegenerative diseases, such as Alzheimer’s, is under investigation, particularly regarding dysregulated mRNA metabolism.
Recombinant human CARHSP1. produced via bacterial expression systems, enables functional studies, revealing its phosphorylation-dependent regulatory mechanisms and interaction partners. Despite advances, CARHSP1's precise molecular mechanisms and therapeutic potential remain areas of active research.
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