| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARD6 |
| Uniprot No | Q9BX69 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-4037aa |
| 氨基酸序列 | MATESTPSEIIERERKKLLEILQHDPDSILDTLTSRRLISEEEYETLENVTDLLKKSRKLLILVQKKGEATCQHFLKCLFSTFPQSAAICGLRHEVLKHENTVPPQSMGASSNSEDAFSPGIKQPEAPEITVFFSEKEHLDLETSEFFRDKKTSYRETALSARKNEKEYDTPEVTLSYSVEKVGCEVPATITYIKDGQRYEELDDSLYLGKEEYLGSVDTPEDAEATVEEEVYDDPEHVGYDGEEDFENSETTEFSGEEPSYEGSETSLSLEEEQEKSIEERKKVFKDVLLCLNMDRSRKVLPDFVKQFSLDRGCKWTPESPGDLAWNFLMKVQARDVTARDSILSHKVLDEDSKEDLLAGVENLEIRDIQTINPLDVLCATMLCSDSSLQRQVMSNMYQCQFALPLLLPDAENNKSILMLGAMKDIVKKQSTQFSGGPTEDTEKFLTLMKMPVISFVRLGYCSFSKSRILNTLLSPAQLKLHKIFLHQDLPLLVLPRQISDGLVEITWCFPDSDDRKENPFFQKPVALANLRGNLESFWTQFGFLMEVSSAVFFFTDCLGEKEWDLLMFLGEAAIERCYFVLSSQARESEEAQIFQRILNLKPAQLLFWERGDAGDRRKNMEGLQAALQEVMFSSCLRCVSVEDMAALARELGIQVDEDFENTQRIQVSSGENMAGTAEGEGQQRHSQLKSSSKSQALMPIQEPGTQCELSQNLQNLYGTPVFRPVLENSWLFPTRIGGNFNHVSLKASWVMGRPFGSEQRPKWFHPLPFQNAGAQGRGKSFGIQSFHPQIFYSGERFMKFSRVARGCHSNGTFGRLPRPICQHVQACPERPQMMGTLERSRAVASKIGHSYSLDSQPARAVGKPWPQQACTRVTELTEATGKLIRTSHIGKPHPQSFQPAAATQKLRPASQQGVQMKTQGGASNPALQIGSHPMCKSSQFKSDQSNPSTVKHSQPKPFHSVPSQPKSSQTKSCQSQPSQTKPSPCKSTQPKPSQPWPPQSKPSQPRPPQPKSSSTNPSQAKAHHSKAGQKRGGKH |
| 分子量 | 116.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CARD6蛋白的3篇参考文献摘要示例(注：以下内容为模拟生成的示例，部分文献可能需要根据实际数据库检索验证)：
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1. **文献名称**: *"CARD6 modulates NF-κB and MAPK signaling in response to bacterial components"*
**作者**: Li, X., et al.
**摘要**: 该研究揭示了CARD6通过其羧酸末端结构域与TAK1结合，调控NF-κB和MAPK炎症信号通路的活化，并在细菌脂多糖(LPS)刺激下增强炎症反应。研究使用重组人CARD6蛋白验证了其与TAK1的相互作用机制。
2. **文献名称**: *"Structural characterization and functional analysis of the CARD6-caspase interaction"*
**作者**: Wang, Y., et al.
**摘要**: 通过X射线晶体学解析重组人CARD6蛋白的结构，发现其N端CARD结构域可与caspase家族成员(如caspase-8)结合，并证明CARD6通过该互作正向调节凋亡信号通路，尤其在肿瘤细胞中表现显著。
3. **文献名称**: *"CARD6 deficiency attenuates inflammation and fibrosis in a murine model of cardiomyopathy"*
**作者**: Zhang, H., et al.
**摘要**: 研究通过构建CARD6基因敲除小鼠模型，发现CARD6缺失会抑制TLR4介导的心脏炎症和纤维化相关蛋白表达，提示重组CARD6可能通过靶向TLR4信号参与心肌病的病理进程。
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如需获取具体文献，建议在 **PubMed** 或 **Google Scholar** 中检索关键词：**"CARD6 protein"**、**"CARD6 signaling"**、**"recombinant CARD6"**。部分研究可能集中在CARD家族的泛素化、细胞死亡调控或感染免疫等领域。
Caspase recruitment domain-containing protein 6 (CARD6) is a member of the CARD family, which plays regulatory roles in innate immune signaling pathways and apoptosis. Structurally, it features an N-terminal CARD domain that facilitates homotypic interactions with other CARD-containing proteins, such as certain caspases and receptor-interacting serine/threonine-protein kinase 2 (RIPK2), and a coiled-coil domain involved in oligomerization. CARD6 functions as a scaffold protein, linking innate immune receptors (e.g., NOD1) to downstream effectors, thereby modulating NF-κB and MAPK activation. Studies suggest its involvement in balancing inflammatory responses and apoptotic signaling, though its precise mechanisms remain incompletely characterized.
Recombinant human CARD6 is engineered for *in vitro* studies to elucidate its interaction networks, enzymatic regulation, and structural properties. Produced via bacterial or mammalian expression systems, it enables biochemical assays, crystallography, and cell-based functional analyses. While CARD6 knockout models show no overt developmental defects, altered immune responses in such models highlight its context-dependent roles in infection and inflammation. Emerging evidence links CARD6 dysregulation to pathologies, including cancers and autoimmune diseases, positioning it as a potential therapeutic target. However, gaps persist in understanding its tissue-specific functions and crosstalk with other signaling hubs (e.g., NLRs, death receptors). Further research on recombinant CARD6 could clarify its physiological relevance and diagnostic/therapeutic potential.
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