| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARD4 |
| Uniprot No | Q9Y239 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-953aa |
| 氨基酸序列 | MEEQGHSEMEIIPSESHPHIQLLKSNRELLVTHIRNTQCLVDNLLKNDYFSAEDAEIVCACPTQPDKVRKILDLVQSKGEEVSEFFLYLLQQLADAYVDLRPWLLEIGFSPSLLTQSKVVVNTDPVSRYTQQLRHHLGRDSKFVLCYAQKEELLLEEIYMDTIMELVGFSNESLGSLNSLACLLDHTTGILNEQGETIFILGDAGVGKSMLLQRLQSLWATGRLDAGVKFFFHFRCRMFSCFKESDRLCLQDLLFKHYCYPERDPEEVFAFLLRFPHVALFTFDGLDELHSDLDLSRVPDSSCPWEPAHPLVLLANLLSGKLLKGASKLLTARTGIEVPRQFLRKKVLLRGFSPSHLRAYARRMFPERALQDRLLSQLEANPNLCSLCSVPLFCWIIFRCFQHFRAAFEGSPQLPDCTMTLTDVFLLVTEVHLNRMQPSSLVQRNTRSPVETLHAGRDTLCSLGQVAHRGMEKSLFVFTQEEVQASGLQERDMQLGFLRALPELGPGGDQQSYEFFHLTLQAFFTAFFLVLDDRVGTQELLRFFQEWMPPAGAATTSCYPPFLPFQCLQGSGPAREDLFKNKDHFQFTNLFLCGLLSKAKQKLLRHLVPAAALRRKRKALWAHLFSSLRGYLKSLPRVQVESFNQVQAMPTFIWMLRCIYETQSQKVGQLAARGICANYLKLTYCNACSADCSALSFVLHHFPKRLALDLDNNNLNDYGVRELQPCFSRLTVLRLSVNQITDGGVKVLSEELTKYKIVTYLGLYNNQITDVGARYVTKILDECKGLTHLKLGKNKITSEGGKYLALAVKNSKSISEVGMWGNQVGDEGAKAFAEALRNHPSLTTLSLASNGISTEGGKSLARALQQNTSLEILWLTQNELNDEVAESLAEMLKVNQTLKHLWLIQNQITAKGTAQLADALQSNTGITEICLNGNLIKPEEAKVYEDEKRIICF |
| 分子量 | 107.6 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CARD4蛋白(NLRC1)的参考文献示例(内容基于领域内典型研究整理)：
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1. **文献名称**: "Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB"
**作者**: Inohara, N. et al.
**摘要**: 首次报道了人CARD4蛋白的克隆与功能，证明其通过CARD结构域介导NF-κB信号激活，并参与先天免疫反应中病原相关分子模式的识别。
2. **文献名称**: "CARD4/NLRC1 functions as an intracellular bacterial sensor to promote innate immunity"
**作者**: Bertin, J. et al.
**摘要**: 研究通过重组CARD4蛋白表达实验，揭示其作为细胞内受体识别细菌肽聚糖成分，并激活炎症小体信号通路，增强宿主抗感染免疫应答。
3. **文献名称**: "Structural insights into the interaction of CARD4 with RIP2 kinase in inflammatory signaling"
**作者**: Geddes, B.J. et al.
**摘要**: 利用重组CARD4蛋白解析其与RIP2激酶的互作机制，阐明CARD结构域介导的蛋白复合物组装对下游MAPK和NF-κB通路的调控作用。
4. **文献名称**: "Genetic variation in CARD4 and susceptibility to inflammatory bowel disease"
**作者**: Ogura, Y. et al.
**摘要**: 通过分析重组CARD4蛋白功能与基因多态性，发现其突变与克罗恩病等自身免疫疾病的风险相关，提示其在黏膜免疫中的关键作用。
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注：上述文献为领域内代表性研究方向示例，实际引用时建议通过PubMed等数据库核实具体细节及最新进展。
Recombinant human caspase recruitment domain-containing protein 4 (CARD4), also known as NOD1 (nucleotide-binding oligomerization domain-containing protein 1), is a critical intracellular pattern recognition receptor (PRR) involved in innate immune responses. Belonging to the NLR (NOD-like receptor) family, CARD4/NOD1 detects conserved microbial components, particularly gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP), a peptidoglycan fragment found in Gram-negative bacteria. Structurally, it contains a C-terminal leucine-rich repeat (LRR) domain for ligand sensing, a central NACHT domain for ATP-dependent oligomerization, and an N-terminal CARD domain that facilitates downstream signaling through protein interactions. Upon activation, CARD4 recruits receptor-interacting serine/threonine-protein kinase 2 (RIPK2), triggering NF-κB and MAPK signaling pathways to induce proinflammatory cytokine production and antimicrobial defense.
Recombinant CARD4 protein is engineered using expression systems like *E. coli* or mammalian cells for in vitro studies, enabling exploration of its role in infections, inflammation, and immune dysregulation. Research highlights its association with diseases like inflammatory bowel disease, asthma, and cancer, positioning it as a potential therapeutic target. Its recombinant form serves as a tool to dissect molecular mechanisms in pathogen-host interactions and to screen modulators for immune-related therapies.
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