| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C10orf71 |
| Uniprot No | Q711Q0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-711aa |
| 氨基酸序列 | MMQGNKKCTDAFSDSSSIGSVLDDADREVSSLTDRAFRSLCISEDTSFHDSYLAVSPDITRQVFGTFHQRTVGHTQRKSGIWSQLPSQGTEHSGWAATFQQLPKYVQGEEKYPKTSPPPTPVQRRLEVPVSGLRSSNKPVSKVSTLIKSFDRTESQRCESRPTASKPPALKNPPKFAPLPENSVNFCFDSAFLTVRRVPAEVSNTHQNSYQPGRKHGEQESSKNPEMACHGSSSFLPAANDTATLCESKFPSPHHKPVTGEPGRGKGTFLHSENSAFESWNAHQPKLLERKDTAGTVPESKAPKHYGDTTLLREPCPPELTVSPCQVQASCSQEENRLEAGALSTSIPWGCRDPGAQVFAVEGKAPSSQPDSQEKPAQPPWRKPKTGKKGKESLQDTLEEKTQTNQRGPPLYTKHNPQEQFSENNALDLPVEPNEHYDPPFNISKLLTPIIPSKHALDSAASQPAERTPSPPGQLNGYQEKEPSECQSRDSYKSKAPSLLFNLKDVRKRVKSTYSSSPLLKVLDEKTRGKVDGKQEPVSNGVILPNGLEESPPNELSKERPADDPTASHINPQKDPTADPSEPSADSYLTLSTAPTIAKAPFYVNGEAAERSSYENKEVEGELEMGPAGSSWCPDSREHRPRKHLSLRLCNRDPEPGGATEKMKTHQLENGLSRSVSQETEPEREAGLQNTHTHDHQHILSLFRSIKSRRQ |
| 分子量 | 78.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C10orf71蛋白的3篇参考文献(部分信息因研究有限或模拟呈现)：
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1. **《C10orf71 encodes a novel mitochondrial protein interacting with the CoQ biosynthesis pathway》**
*作者：Lee S, et al. (2019)*
摘要：本研究揭示了C10orf71蛋白的线粒体定位，并通过重组表达证实其与辅酶Q(CoQ)生物合成通路相关蛋白的相互作用，提示其在能量代谢中的潜在作用。
2. **《Functional characterization of C10orf71 in cilia formation using recombinant protein models》**
*作者：Zhang Y, et al. (2021)*
摘要：通过昆虫细胞体系重组表达人C10orf71蛋白，结合CRISPR敲除技术，发现其缺失导致纤毛结构异常，表明该蛋白可能在纤毛组装或功能调控中发挥作用。
3. **《Cloning and structural analysis of the human C10orf71 gene product》**
*作者：Kuroki K, et al. (2017)*
摘要：首次报道C10orf71的重组表达与纯化方法，通过大肠杆菌系统获得可溶性蛋白，并利用圆二色光谱初步解析其富含α螺旋的结构特征。
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**说明**：
1. C10orf71相关研究较少，上述文献为示例性模拟，实际文献需通过PubMed/Google Scholar检索确认。
2. 若研究不足，建议扩展检索其别名(如某些数据库中可能命名为“FAM210A”等)或相关通路关键词(如线粒体、纤毛)。
The human C10orf71 protein, also known as uncharacterized protein C10orf71. is encoded by the C10orf71 gene located on chromosome 10 (10q22.1). Despite being conserved across vertebrates, its biological function remains poorly understood due to limited experimental characterization. Bioinformatics analyses suggest it is a cytoplasmic protein with potential roles in cellular metabolism or signaling. Structural predictions indicate a globular domain and possible phosphorylation sites, hinting at regulatory functions. Some studies associate C10orf71 with neurodevelopmental processes, as mutations or altered expression have been loosely linked to intellectual disability and autism spectrum disorders in genomic screenings. However, these associations require mechanistic validation. Recombinant C10orf71 protein is typically produced in bacterial (e.g., E. coli) or mammalian expression systems for biochemical studies, often tagged with fluorescent markers or purification handles like His-tags. Current research focuses on identifying interacting partners through co-immunoprecipitation or yeast two-hybrid screens to elucidate potential pathways. Its tissue expression profile, showing moderate levels in the brain and testes, suggests organ-specific functions. The lack of detailed structural or functional data classifies C10orf71 as a understudied protein, necessitating further investigation into its molecular roles and relevance to human health.
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