| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C10orf53 |
| Uniprot No | Q8N6V4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-157aa |
| 氨基酸序列 | MPKNAVVILRYGPYSAAGLPVEHHTFRLQGLQAVLAIDGHEVILEKIEDWNVVELMVNEEVIFHCNIKDLEFGKLTPSSDKRTTSSSRLTFHQLSSPCGMKVSPLQQFPQKTQDLTCIVLAQIGSCIHFQTNLCDLGWPGLDHMLISGLEKRGTQPY |
| 分子量 | 44 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人C10orf53蛋白的参考文献示例(注：C10orf53研究相对有限，以下信息可能部分基于领域内相关推测，请结合具体数据库验证)：
1. **文献名称**: *"Identification and functional characterization of C10orf53 as a novel cancer-associated protein"*
**作者**: Smith A, et al.
**摘要**: 研究首次报道C10orf53在结直肠癌组织中高表达，并通过重组蛋白实验证明其可促进细胞增殖和迁移。机制研究表明其可能通过调控Wnt/β-catenin通路发挥作用。
2. **文献名称**: *"Structural insights into the DNA-binding activity of C10orf53 through recombinant expression and crystallography"*
**作者**: Chen L, et al.
**摘要**: 通过重组表达纯化人C10orf53蛋白并解析其晶体结构，揭示其具有潜在的DNA结合域。体外实验证实其与特定启动子区域结合，提示其在基因调控中的潜在作用。
3. **文献名称**: *"C10orf53 expression profile and its role in cellular stress response"*
**作者**: Zhang Y, et al.
**摘要**: 利用重组C10orf53蛋白进行功能研究，发现其在氧化应激条件下表达上调。敲低该蛋白可增加细胞凋亡率，提示其可能参与氧化应激保护机制。
4. **文献名称**: *"Proteomic analysis identifies C10orf53 as a biomarker for early-stage breast cancer"*
**作者**: Wang H, et al.
**摘要**: 基于重组C10orf53的抗体检测临床样本，发现该蛋白在乳腺癌患者血清中显著升高，可能作为早期诊断标志物。
**注意**：以上文献标题及内容为假设示例，具体文献需通过PubMed、Google Scholar等平台以“C10orf53”或“Chromosome 10 open reading frame 53”为关键词检索。若研究较少，建议扩展至其可能的别名或功能关联基因(如TP53相关通路研究)。
The human protein encoded by the *C10orf53* gene (Chromosome 10 Open Reading Frame 53), also termed **Rosslyn syndrome critical region protein 1** (RSC1A1), remains poorly characterized but has attracted interest due to its potential roles in cellular metabolism and disease. Identified through genomic sequencing projects, this small protein (~20 kDa) is highly conserved across vertebrates, suggesting essential biological functions. It is broadly expressed in tissues, particularly in the kidney, liver, and pancreas.
Studies indicate C10orf53 may regulate ion transport or nutrient sensing pathways, with proposed interactions involving sodium-glucose co-transporters (SGLTs) or other membrane proteins. However, its molecular mechanisms remain unclear. Intriguingly, genetic variants near *C10orf53* are associated with metabolic disorders, including type 2 diabetes and hypertension, though causal links require validation. A frameshift mutation in *C10orf53* was linked to Rosslyn syndrome, a rare condition featuring renal and pancreatic dysfunction.
Recombinant C10orf53 protein (rC10orf53) is typically produced in *E. coli* or mammalian systems for functional studies. Its structural simplicity (no known transmembrane domains or post-translational modifications) facilitates purification. Current research focuses on clarifying its physiological substrates, interactome, and potential as a therapeutic target. Limited commercial antibodies and tools hinder progress, emphasizing the need for deeper investigation into this enigmatic protein.
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