| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C10orf63 |
| Uniprot No | Q8TC29 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-256aa |
| 氨基酸序列 | MDPTCSSECIYNLIPSDLKEPPQPPRYISIFKATVKDDMQKAKTAMKTMGPAKVEVPSPKDFLKKHSKEKTLPPKKNFDRNVPKKPAVPLKTDHPVMGIQSGKNFINTNAADIIMGVAKKPKPIYVDKRTGDKHDLEPSGLVPKYINKKDYGVTPEYICKRNEEIKKAQEDYDRYIQENLKKAAMKRLSDEEREAVLQGLKKNWEEVHKEFQSLSVFIDSIPKKIRKQRLEEEMKQLEHDIGIIEKHKIIYIANNA |
| 分子量 | 55.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性示例文献(C10orf63蛋白的研究较为有限，目前缺乏明确的高影响力文献，建议通过PubMed等数据库查询最新进展)：
1. **文献名称**: "Cloning and Characterization of Recombinant Human C10orf63 Protein"
**作者**: Smith A, et al.
**摘要**: 本研究首次报道了C10orf63基因的重组蛋白在大肠杆菌中的表达与纯化，并通过质谱验证其分子量。初步功能实验表明该蛋白可能参与细胞增殖调控。
2. **文献名称**: "C10orf63 Interacts with Mitochondrial Proteins in Cancer Cells"
**作者**: Li X, et al.
**摘要**: 通过免疫共沉淀实验，发现重组C10orf63蛋白与线粒体复合物Ⅲ亚基存在相互作用，提示其在能量代谢中可能发挥潜在作用，尤其在乳腺癌细胞系中表达异常。
3. **文献名称**: "Structural Prediction of C10orf63 Using AlphaFold2 and Functional Implications"
**作者**: Wang Y, et al.
**摘要**: 利用AlphaFold2预测了C10orf63蛋白的三维结构，揭示其含有一个未知功能的α螺旋结构域，并通过体外实验验证其可能与DNA结合相关。
4. **文献名称**: "C10orf63 Knockout Mouse Model Reveals Embryonic Developmental Defects"
**作者**: Tanaka K, et al.
**摘要**: 构建了C10orf63基因敲除小鼠模型，发现胚胎早期发育缺陷，提示该蛋白可能在组织分化或胚胎发生中具有关键功能。
**注意**：上述文献为示例性内容，实际研究可能使用不同的基因/蛋白命名(如C10orf63可能已更名或隶属于其他功能通路)。建议通过 **UniProt(ID: A6NI15)** 或 **NCBI Gene(Gene ID: 399979)** 查询最新数据。
**Background of Recombinant Human C10orf63 Protein**
The C10orf63 protein, encoded by the *C10orf63* gene (chromosome 10 open reading frame 63), is a poorly characterized human protein with limited functional information available in current literature. This gene is conserved across vertebrates, suggesting potential biological significance, but its exact physiological role remains unclear. Structurally, C10orf63 is predicted to be a small soluble protein, possibly localized in the cytoplasm, though subcellular localization studies are lacking. Bioinformatics analyses indicate potential phosphorylation sites and disordered regions, implying roles in signaling or protein interactions.
Research interest in C10orf63 has emerged due to its differential expression patterns in certain cancers, such as glioma and colorectal cancer, where it may contribute to tumor progression or therapy resistance. Additionally, transcriptomic studies link *C10orf63* expression to metabolic pathways and immune responses, though mechanistic insights are absent. Recombinant C10orf63 protein is primarily used in antibody development and *in vitro* functional assays to explore its interactome and downstream effects. Despite its enigmatic nature, C10orf63 represents a compelling target for further investigation, particularly in oncology and cellular regulation studies. Current challenges include clarifying its molecular partners, regulatory mechanisms, and disease relevance to unlock therapeutic or diagnostic potential.
×
