| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | KRT2; KRT2A; KRT2E; Keratin, type II cytoskeletal 2 epidermal; Cytokeratin-2e; CK-2e; Epithelial keratin-2e; Keratin-2 epidermis; Keratin-2e; K2e; Type-II keratin Kb2; KRT76; KRT2B; KRT2P; Keratin, type II cytoskeletal 2 oral; Cytokeratin-2P |
| Entrez GeneID | 3849; |
| WB Predicted band size | 65kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Cytokeratin around the acetylation site of K194. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于Cytokeratin-pan (Acetyl-Lys194)抗体的虚构参考文献示例(请注意，实际文献可能需要通过学术数据库验证)：
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1. **文献名称**: "Acetylation of cytokeratin at Lys194 regulates epithelial cell migration"
**作者**: Smith A, et al.
**摘要**: 利用Cytokeratin-pan (Acetyl-Lys194)抗体，通过免疫荧光和蛋白质印迹分析，揭示了Lys194位点乙酰化通过调控细胞角蛋白网络稳定性，影响上皮细胞的迁移和伤口愈合过程。
2. **文献名称**: "Post-translational modifications of pan-cytokeratin in cancer progression"
**作者**: Zhang Y, et al.
**摘要**: 研究泛细胞角蛋白在多种癌症中的翻译后修饰模式，发现Lys194乙酰化(通过特异性抗体检测)与肿瘤侵袭性相关，可能作为潜在的生物标志物。
3. **文献名称**: "Characterization of acetylation-dependent cytokeratin interactions"
**作者**: Lee H, et al.
**摘要**: 结合质谱和Cytokeratin-pan (Acetyl-Lys194)抗体，系统鉴定了乙酰化修饰对细胞角蛋白与其他细胞骨架蛋白相互作用的调控机制，提示其在细胞力学响应中的功能。
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**备注**：以上文献为示例性质，实际研究中可能需要通过PubMed、Google Scholar等平台以关键词“Cytokeratin pan Acetyl-Lys194”或“cytokeratin acetylation site-specific”检索真实文献。若该抗体为商业化产品，建议参考供应商提供的产品说明书及相关引用文献。
**Background of Cytokeratin-pan (Acetyl-Lys194) Antibody**
Cytokeratins are intermediate filament proteins primarily expressed in epithelial cells, forming a critical structural framework that maintains cellular integrity and facilitates mechanical stress resistance. They are classified into type I (acidic) and type II (neutral-basic) subtypes, which assemble into heteropolymers. The "pan" designation indicates that the antibody recognizes a broad spectrum of cytokeratin isoforms, making it a versatile tool for identifying epithelial-derived cells or tissues.
The **Acetyl-Lys194** epitope specifically highlights acetylation at lysine 194. a post-translational modification (PTM) within the conserved rod domain of certain cytokeratins. Acetylation modulates cytokeratin function by influencing filament assembly, stability, and interactions with other proteins. This modification is implicated in cellular processes such as differentiation, apoptosis, and stress responses, and may serve as a biomarker in pathological conditions like cancer or epithelial injury.
The Cytokeratin-pan (Acetyl-Lys194) antibody is widely used in immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to study epithelial cell behavior, tumor progression, and metastasis. Its ability to detect acetylated cytokeratins provides insights into epigenetic regulation of the cytoskeleton, particularly in epithelial-mesenchymal transition (EMT) or drug resistance mechanisms. Researchers also employ this antibody to distinguish epithelial-derived cancers (e.g., carcinomas) from other malignancies, aiding in diagnostics and therapeutic targeting.
Overall, this antibody bridges structural biology and disease research, emphasizing the role of PTMs in cytokeratin dynamics and epithelial pathology.
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