Rabbit Polyclonal HNF-3α/β/γ(Acetyl-Lys264/253/211) Antibody

The HNF-3α/β/γ (Acetyl-Lys264/253/211) antibody is designed to detect the acetylated forms of Hepatocyte Nuclear Factor 3 (HNF-3), also known as Forkhead Box A (FoxA) transcription factors (FoxA1/A2/A3 for α/β/γ, respectively). These evolutionarily conserved proteins play critical roles in organ development, glucose metabolism, and tissue-specific gene regulation, particularly in the liver, pancreas, and nervous system. Acetylation at conserved lysine residues (Lys264 in FoxA1/α, Lys253 in FoxA2/β, and Lys211 in FoxA3/γ) is a key post-translational modification that modulates their DNA-binding affinity, transcriptional activity, and interactions with co-regulators. This acetylation event, often mediated by histone acetyltransferases like CBP/p300. enhances chromatin accessibility and promotes gene activation. The antibody specifically recognizes the acetylated states of these residues, enabling researchers to study dynamic regulatory mechanisms in cellular differentiation, metabolic homeostasis, and disease contexts such as diabetes, obesity, and cancer. Its applications span Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence, providing insights into FoxA protein function and epigenetic regulation.