| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha |
| Entrez GeneID | 3419; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IDH3 alpha. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"IDH3α regulates one-carbon metabolism in glioblastoma"**
*Authors: Li et al.*
**摘要**：研究利用IDH3α抗体分析胶质母细胞瘤中IDH3α蛋白表达，发现其通过调控一碳代谢影响肿瘤生长，为治疗提供了新靶点。
2. **"Antibody-based detection of mitochondrial IDH3α in colorectal cancer"**
*Authors: Smith et al.*
**摘要**：开发高特异性IDH3α抗体，验证其在结直肠癌组织中线粒体定位，揭示IDH3α表达缺失与患者预后不良相关。
3. **"IDH3α mutations disrupt enzyme activity in neurodegenerative disorders"**
*Authors: Garcia-Ruiz et al.*
**摘要**：通过IDH3α抗体检测突变蛋白稳定性，发现突变导致酶活性下降，与线粒体功能障碍和神经退行性疾病相关。
4. **"Characterization of a monoclonal antibody against human IDH3α for metabolic studies"**
*Authors: Tanaka et al.*
**摘要**：报道一种抗人IDH3α单克隆抗体的制备及验证，应用于Western blot和免疫荧光，揭示其在缺氧条件下的表达变化。
(注：上述文献为示例性质，实际引用需以真实发表文章为准。)
The IDH3 alpha (IDH3α) antibody is a crucial tool in studying the function and expression of isocitrate dehydrogenase 3 alpha, a subunit of the IDH3 enzyme complex. IDH3 is a key metabolic enzyme in the tricarboxylic acid (TCA) cycle, catalyzing the oxidative decarboxylation of isocitrate to α-ketoglutarate while generating NADH. Unlike its isoforms IDH1 and IDH2. which are frequently mutated in cancers (e.g., gliomas, AML), IDH3α mutations are rare but have been implicated in certain metabolic disorders and cancer subtypes. The IDH3α subunit, encoded by the IDH3A gene, forms the catalytic core of the heterotrimeric IDH3 complex (α/β/γ), essential for mitochondrial energy production.
Antibodies targeting IDH3α are widely used in research to investigate its expression patterns, subcellular localization (mitochondrial), and dysregulation in diseases. These antibodies are validated for applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Given the structural similarities among IDH family members, high specificity is critical for distinguishing IDH3α from IDH1/IDH2. Some antibodies are designed to detect post-translational modifications or mutations linked to functional alterations. In cancer research, IDH3α antibodies help explore metabolic reprogramming ("Warburg effect") and potential therapeutic targets. Additionally, they aid in studying rare IDH3A-associated genetic disorders, such as retinitis pigmentosa and enzyme deficiency syndromes. Proper validation using knockout controls or recombinant proteins is essential to ensure antibody reliability, as off-target binding can complicate interpretation.
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