| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 28S ribosomal protein S33, mitochondrial; MRP-S33; S33mt |
| Entrez GeneID | 51650; |
| WB Predicted band size | 12kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MRPS33. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRPS33抗体的3篇虚构参考文献示例，展示了可能的文献结构和内容：
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1. **文献名称**：*MRPS33在结直肠癌中的异常表达及其临床意义*
**作者**：Zhang A, et al.
**摘要**：本研究通过免疫组化及Western blot分析，利用MRPS33特异性抗体发现其在结直肠癌组织中显著高表达，并与患者预后不良相关。功能实验表明，MRPS33通过调控线粒体呼吸链活性促进肿瘤细胞增殖。
2. **文献名称**：*MRPS33基因敲除对线粒体功能的影响*
**作者**：Li B, et al.
**摘要**：通过CRISPR技术构建MRPS33敲除细胞模型，结合抗体验证蛋白表达缺失。研究发现，MRPS33缺失导致线粒体核糖体组装异常，呼吸链复合物活性降低，最终引发细胞凋亡。
3. **文献名称**：*一种高灵敏度MRPS33单克隆抗体的开发与验证*
**作者**：Wang C, et al.
**摘要**：报道了一种新型MRPS33单克隆抗体的制备方法，该抗体在ELISA、免疫荧光和流式细胞术中表现优异，为研究MRPS33在疾病中的分子机制提供了可靠工具。
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**说明**：以上文献为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“MRPS33 antibody”“mitochondrial ribosomal protein S33”)获取。建议结合具体研究方向筛选相关论文。
The mitochondrial ribosomal protein S33 (MRPS33) is a component of the small subunit (28S) of the mitochondrial ribosome (mitoribosome), which is essential for synthesizing proteins encoded by mitochondrial DNA. These proteins are critical for oxidative phosphorylation (OXPHOS), the primary ATP-producing pathway in eukaryotic cells. MRPS33 plays a structural and functional role in mitoribosome assembly and stability, ensuring efficient translation of mitochondrial mRNAs. Mutations or dysregulation of MRPS33 have been linked to mitochondrial disorders, metabolic defects, and cancer, as impaired mitochondrial protein synthesis disrupts cellular energy metabolism and promotes disease progression.
MRPS33 antibodies are tools used to study its expression, localization, and interactions in cellular models. They are commonly produced in rabbits or mice using recombinant MRPS33 protein or synthetic peptides as immunogens. Validated applications include Western blotting, immunofluorescence, and immunohistochemistry to assess MRPS33 levels in tissues or cultured cells, particularly in studies of mitochondrial dysfunction. Specificity is confirmed via knockdown/knockout controls or immunoprecipitation assays. These antibodies also aid in exploring MRPS33's role in pathologies, such as tumorigenesis (e.g., altered expression in certain cancers) or rare mitochondrial diseases, and its interaction with other mitoribosomal proteins. Researchers leverage MRPS33 antibodies to uncover mechanisms linking mitochondrial translation defects to cellular stress, apoptosis, or metabolic reprogramming.
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