Rabbit Polyclonal NT5C3 Antibody

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     Western blot analysis of MRPS7 expression in rat tongue whole cell lysates.    

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     Immunofluorescent analysis of NT5C3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.    

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     Immunohistochemical analysis of NT5C3 staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.    

The NT5C3 antibody is a crucial tool for studying the NT5C3 protein, also known as cytosolic 5'-nucleotidase III (cN-III) or cytidine deaminase-like protein. NT5C3 belongs to the 5'-nucleotidase family, which regulates intracellular nucleotide pools by hydrolyzing nucleoside monophosphates into nucleosides and inorganic phosphate. This enzyme plays a role in maintaining nucleotide balance, influencing DNA/RNA synthesis and the activation of nucleoside analog drugs. Dysregulation of NT5C3 has been linked to hematologic disorders, such as congenital hemolytic anemia due to mutations affecting erythrocyte nucleotide metabolism, and cancer, where overexpression may contribute to chemoresistance in leukemia or solid tumors.

The NT5C3 antibody enables researchers to detect and quantify NT5C3 expression in cells and tissues using techniques like Western blotting, immunohistochemistry, and flow cytometry. Its applications span investigating disease mechanisms, drug response pathways, and metabolic dysfunctions. In cancer research, NT5C3 is studied for its potential role in promoting resistance to antimetabolite therapies like gemcitabine or 5-fluorouracil. Additionally, the antibody aids in exploring genetic disorders tied to nucleotide pool imbalances, offering insights into therapeutic targets. By facilitating precise protein localization and expression analysis, the NT5C3 antibody supports advances in understanding cellular metabolism and developing targeted treatments.