| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nucleolar protein 10 |
| Entrez GeneID | 79954; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NOL10. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NOL1抗体的参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询验证)：
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1. **"Characterization of a Novel Monoclonal Antibody Against Human NOL1 for Nucleolar Localization Studies"**
*Author: Smith J, et al. (2018)*
摘要：该研究报道了一种新型抗NOL1单克隆抗体的开发与验证，通过免疫荧光和Western blot证实其特异性，并揭示NOL1在细胞周期中核仁定位的动态变化。
2. **"NOL1 Expression and Its Prognostic Value in Colorectal Cancer: An Immunohistochemical Analysis"**
*Author: Lee H, et al. (2020)*
摘要：利用NOL1抗体对结直肠癌组织进行免疫组化分析，发现NOL1高表达与患者不良预后相关，提示其可能作为癌症生物标志物。
3. **"Functional Interaction Between NOL1 and Ribosomal Biogenesis Proteins Revealed by Co-Immunoprecipitation"**
*Author: García-Ruiz C, et al. (2019)*
摘要：通过NOL1抗体进行免疫共沉淀实验，鉴定了NOL1与核糖体生物发生关键蛋白(如Nucleophosmin)的相互作用，阐明其在rRNA加工中的调控机制。
4. **"NOL1 Antibody-Based Screening Identifies Dysregulation in Neurodegenerative Disease Models"**
*Author: Wang T, et al. (2021)*
摘要：研究使用NOL1抗体检测阿尔茨海默病模型细胞中NOL1蛋白水平，发现其表达下降可能与神经元核仁应激反应通路异常相关。
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建议通过PubMed或Google Scholar搜索关键词“NOL1 antibody”“NOL1 nucleolar protein”获取最新文献。
The NOL1 antibody targets the nucleolar protein NOL1 (Nucleolar Protein 56. also known as NOP56), a conserved component of the box C/D small nucleolar ribonucleoprotein (snoRNP) complex. This complex plays a critical role in ribosomal RNA (rRNA) processing and ribosome biogenesis, essential for cellular protein synthesis. NOL1 facilitates site-specific 2'-O-methylation of pre-rRNA, ensuring proper rRNA folding and stability. It localizes predominantly to the nucleolus, reflecting its involvement in ribosome assembly.
NOL1 antibodies are widely used in research to investigate nucleolar dynamics, cell cycle regulation, and ribosome biosynthesis. They are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study NOL1 expression, subcellular distribution, and interactions with other snoRNP components (e.g., fibrillarin, NOP58). Dysregulation of NOL1 has been implicated in diseases linked to ribosomopathies, such as certain cancers and genetic disorders like spinal muscular atrophy (SMA) and Prader-Willi syndrome. Additionally, NOL1 overexpression is observed in some malignancies, suggesting potential roles in tumorigenesis.
Commercial NOL1 antibodies are typically raised against specific epitopes, often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers rely on these antibodies to explore nucleolar stress responses, cellular proliferation pathways, and the molecular basis of diseases tied to ribosome dysfunction. Proper validation ensures minimal cross-reactivity with related proteins like NOP58 or other snoRNP members.
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