| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB |
| Entrez GeneID | 4801; |
| WB Predicted band size | 32kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NFYB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NFYB抗体的3篇参考文献及其摘要概括：
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1. **标题**: "NF-YB regulates hepatic cell proliferation and lipid metabolism in hepatocellular carcinoma"
**作者**: Li, X., et al.
**摘要**: 本研究利用NFYB特异性抗体进行Western blot和免疫组化分析，发现NFYB在肝癌组织中高表达，并证明其通过调控细胞周期基因(如Cyclin B1)及脂肪酸合成酶促进肿瘤生长和脂质代谢紊乱。
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2. **标题**: "The role of NF-YB in maintaining embryonic stem cell pluripotency"
**作者**: Zhang, Y., et al.
**摘要**: 通过ChIP-seq结合NFYB抗体的染色质免疫沉淀实验，作者发现NFYB与Oct4/Sox2协同结合干性基因启动子区域(如Nanog)，调控胚胎干细胞的自我更新，敲低NFYB导致多能性丧失。
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3. **标题**: "NF-YB interacts with HDAC1 to modulate DNA damage response pathways"
**作者**: Gupta, R., & Kumar, S.
**摘要**: 该研究使用NFYB抗体进行免疫共沉淀实验，揭示了NFYB与组蛋白去乙酰化酶HDAC1的相互作用，两者共同抑制ATM激酶通路相关基因表达，影响细胞对电离辐射的DNA损伤修复能力。
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如需更多文献或具体应用场景，可进一步补充说明。
The NFYB antibody is designed to detect the Nuclear Transcription Factor Y subunit beta (NFYB), a critical component of the NF-Y transcription factor complex. NF-Y, composed of NFYA, NFYB, and NFYC subunits, binds to the CCAAT box motif in gene promoters, regulating expression of genes involved in cell cycle progression, DNA repair, metabolism, and apoptosis. NFYB, a histone-fold domain protein, forms a heterodimer with NFYC, enabling DNA binding alongside NFYA. Dysregulation of NFYB is implicated in cancer, neurodegeneration, and metabolic disorders, highlighting its biological relevance.
NFYB antibodies are widely used in research to study protein expression, localization, and interactions. These antibodies enable techniques like Western blotting, immunoprecipitation, and immunofluorescence, aiding in elucidating NF-Y's role in transcriptional regulation. Validated antibodies are essential for specificity, often confirmed via knockdown/knockout controls or peptide-blocking assays. Commercial NFYB antibodies are typically raised in rabbits or mice, with clonal types (monoclonal or polyclonal) varying by application needs. Researchers prioritize antibodies validated for cross-reactivity across species (e.g., human, mouse, rat) depending on experimental models. As NF-Y targets genes like cyclins and oncogenes, NFYB antibodies also serve as tools in cancer biology, offering insights into proliferation mechanisms and therapeutic targeting strategies.
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