| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C19orf49; EGFL4; KIAA0817; Multiple epidermal growth factor-like domains protein 8; Multiple EGF-like domains protein 8; Epidermal growth factor-like protein 4; EGF-like protein 4 |
| Entrez GeneID | 1954; |
| WB Predicted band size | 255kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MEGF8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MEGF8抗体的3篇代表性文献的简要总结(基于已有研究推测，仅供参考)：
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1. **文献名称**: *MEGF8 mutations cause Carpenter syndrome by impairing Hedgehog and canonical Wnt signaling*
**作者**: Twigg SRF, et al.
**摘要**: 本研究揭示了MEGF8基因突变通过干扰Hedgehog(Shh)和Wnt信号通路导致Carpenter综合征的机制。作者开发了特异性MEGF8抗体，证实其在患者成纤维细胞中表达降低，并验证了抗体在免疫沉淀和亚细胞定位中的应用。
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2. **文献名称**: *Functional analysis of MEGF8 protein interactions in craniofacial development*
**作者**: Smith J, et al.
**摘要**: 研究利用CRISPR/Cas9敲除模型和MEGF8抗体，发现其与纤毛蛋白复合物的相互作用对颅面发育至关重要。抗体通过免疫组化显示MEGF8在胚胎小鼠神经管中的动态表达模式，支持其在发育调控中的作用。
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3. **文献名称**: *Antibody-based profiling of MEGF8 in ciliopathy models*
**作者**: Johnson KA, et al.
**摘要**: 该研究报道了一种新型兔源多克隆MEGF8抗体的开发与验证，用于斑马鱼和小鼠疾病模型。通过Western blot和免疫荧光，发现MEGF8在纤毛基部富集，其缺失导致纤毛结构异常，提示其在细胞信号转导中的关键功能。
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**备注**：以上文献信息为模拟示例，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“MEGF8 antibody”、“MEGF8 signaling”或“MEGF8 gene function”查找最新研究。
The MEGF8 (Multiple Epidermal Growth Factor-like Domains 8) antibody is a tool used to study the MEGF8 protein, encoded by the *MEGF8* gene located on human chromosome 19q13.2. MEGF8 is a transmembrane protein containing multiple epidermal growth factor (EGF)-like repeats and a laminin globular (LamG) domain, suggesting roles in cell adhesion, signaling, and tissue development. It interacts with proteins involved in planar cell polarity (PCP) and ciliogenesis, notably functioning as a chaperone for the frizzled receptor in the Wnt/PCP pathway. Dysregulation of MEGF8 is linked to Carpenter syndrome (a ciliopathy with craniofacial, skeletal, and cardiac anomalies) and other developmental disorders.
MEGF8 antibodies, typically monoclonal or polyclonal, enable detection of the protein in applications like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate its expression patterns, subcellular localization (e.g., Golgi or ciliary compartments), and interactions in developmental models or disease contexts. Validation often includes knockout controls to confirm specificity. Research using these antibodies contributes to understanding MEGF8's role in embryogenesis, tissue patterning, and ciliary function, with potential implications for diagnosing or treating ciliopathies and congenital syndromes.
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