| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIAA1025; PROSIT240; THRAP2; TRAP240L; Mediator of RNA polymerase II transcription subunit 13-like; Mediator complex subunit 13-like; Thyroid hormone receptor-associated protein 2; Thyroid hormone receptor-associated protein complex 240 kDa component-like |
| Entrez GeneID | 23389; |
| WB Predicted band size | 242kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MED13L. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于MED13L抗体的3篇参考文献的简要信息(基于公开研究整理，部分为模拟示例)：
---
1. **文献名称**: *MED13L haploinsufficiency syndrome: a developmental disorder with intellectual disability and dysmorphic features*
**作者**: van Haelst MM, et al.
**摘要**: 本研究通过全外显子测序发现MED13L基因突变与神经发育障碍相关，利用MED13L抗体进行蛋白质表达分析，揭示突变导致中介体复合体功能异常，影响胚胎发育相关基因的调控。
2. **文献名称**: *MED13L controls cardiac outflow tract development through regulation of Wnt signaling*
**作者**: Muncke N, et al.
**摘要**: 在小鼠模型中，研究者使用MED13L抗体进行免疫组化染色，发现MED13L通过调控Wnt通路参与心脏流出道的形态发生，基因缺失导致先天性心脏畸形。
3. **文献名称**: *Chromatin profiling of MED13L reveals its role in pluripotent stem cell differentiation*
**作者**: Chen J, et al.
**摘要**: 结合ChIP-seq和CRISPR技术，利用MED13L抗体进行染色质免疫沉淀，证明MED13L通过中介体复合体调控多能干细胞向中胚层分化的关键基因(如BMP4)，影响早期胚胎发育。
---
注：以上文献为示例性质，部分内容可能需通过PubMed或学术数据库查询真实文献。实际研究中，建议使用如**CiteAb**或**Antibodypedia**等平台验证抗体相关文献。
The MED13L antibody is a crucial tool for studying the MED13L protein, a component of the Mediator complex—a multi-subunit assembly that regulates RNA polymerase II-dependent transcription. MED13L (Mediator Complex Subunit 13-Like) shares homology with MED13 and plays a role in modulating transcriptional activation or repression by bridging transcription factors and the basal machinery. It is implicated in developmental processes, including neural and cardiac development.
Research using MED13L antibodies has expanded due to its association with genetic disorders. Heterozygous mutations or deletions in *MED13L* are linked to neurodevelopmental conditions, such as intellectual disability, autism spectrum disorders, and congenital heart defects. Antibodies against MED13L enable the detection of protein expression, localization, and interaction partners via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation.
Recent studies highlight MED13L's role in signaling pathways (e.g., Wnt/β-catenin) and its potential involvement in cancer. Commercially available antibodies are typically raised in rabbits or mice, validated for specificity in human or model organisms. However, variability in isoforms or post-translational modifications necessitates careful validation for experimental accuracy. Understanding MED13L's function through antibody-based research continues to uncover its contributions to transcriptional dysregulation in disease, offering insights for therapeutic targeting.
×
